Photoinduced apoptosis using a peptide carrying a photosensitizer

Kazunori Watanabe, Hayato Fujiwara, Mizuki Kitamatsu, Takashi Ohtsuki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A novel molecule, TatBim-Alexa, consisting of the HIV1 Tat cell-penetrating peptide, the Bim apoptosis-inducing peptide, and Alexa Fluor 546 was synthesized for photoinducion of apoptosis. The Alexa Fluor 546 was used as a photosensitizer and covalently attached at the C-terminus of TatBim peptide by the thiol-maleimide reaction. Photo-dependent cytosolic internalization of TatBim-Alexa and photo-dependent apoptosis using TatBim-Alexa were demonstrated in several kinds of mammalian cells including human cancer cell lines.

Original languageEnglish
Pages (from-to)3115-3118
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume26
Issue number13
DOIs
Publication statusPublished - Jul 1 2016

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Keywords

  • Apoptosis
  • Bim
  • Cell-penetrating peptide
  • Photosensitizer

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Molecular Medicine
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

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