Photoelectric dye-coupled polyethylene film as a prototype of retinal prostheses

Akihito Uji, Toshihiko Matsuo, Sanae Ishimaru, Akiko Kajiura, Kaoru Shimamura, Hiroshi Ohtsuki, Yasufumi Dan-Oh, Sadaharu Suga

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Photoelectric dyes, which absorb light and convert photon energy to electric potentials, have been previously shown to stimulate retinal neurons in culture. In this study, a photoelectric dye was coupled to a polyethylene film surface and tested in vitro using retinal tissues from chick embryos at the 12-day embryonic stage, at which time outer segments of retinal photoreceptor cells have not yet developed. Carboxyl moieties were introduced to a polyethylene film surface by fuming nitric acid, and then a photoelectric dye, 2-[2-[4-(dibutylamino)phenyl]ethenyl]-3-carboxymethylbenzothiazolium bromide, was coupled to the film through amide linkage. Intracellular calcium elevation was observed with Fluo-4 in retinal tissues placed on the dye-coupled polyethylene film, in contrast to retinal tissues which had no contact with the film. The response was inhibited by calcicludine, a voltage-gated calcium channel blocker, and also by extracellular calcium depletion. The photoelectric dye, coupled to the polyethylene film surface, absorbed light under a dissecting microscope and stimulated neurons in retinal tissues, showing that the dye-coupled film could be used as a prototype of retinal prostheses.

Original languageEnglish
Pages (from-to)53-57
Number of pages5
JournalArtificial Organs
Volume29
Issue number1
DOIs
Publication statusPublished - Jan 2005

Keywords

  • Chick retina
  • Fluo-4
  • Intracellular calcium
  • Photoelectric dye
  • Pigment
  • Polyethylene film
  • Retinal implant
  • Retinal prosthesis

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

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