Photocurrent Generation of Reconstituted Photosystem II on a Self-Assembled Gold Film

Mariko Miyachi, Shu Ikehira, Daiki Nishiori, Yoshinori Yamanoi, Masato Yamada, Masako Iwai, Tatsuya Tomo, Suleyman Allakhverdiev, Hiroshi Nishihara

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Photosystem II (PSII)-modified gold electrodes were prepared by the deposition of PSII reconstituted with platinum nanoparticles (PtNPs) on Au electrodes. PtNPs modified with 1-[15-(3,5,6-trimethyl-1,4-benzoquinone-2-yl)]pentadecyl disulfide ((TMQ(CH2)15S)2) were incorporated into the QB site of PSII isolated from thermophilic cyanobacterium Thermosynechococcus elongatus. The reconstitution was confirmed by QA-reoxidation measurements. PSII reconstituted with PtNPs was deposited and integrated on a Au(111) surface modified with 4,4′-biphenyldithiol. The cross section of the reconstituted PSII film on the Au electrode was investigated by SEM. Absorption spectra showed that the surface coverage of the electrode was about 18 pmol PSII cm-2. A photocurrent density of 15 nAcm-2 at E = +0.10 V (vs Ag/AgCl) was observed under 680 nm irradiation. The photoresponse showed good reversibility under alternating light and dark conditions. Clear photoresponses were not observed in the absence of PSII and molecular wire. These results supported the photocurrent originated from PSII and moved to a gold electrode by light irradiation, which also confirmed conjugation with orientation through the molecular wire.

Original languageEnglish
Pages (from-to)1351-1358
Number of pages8
JournalLangmuir
Volume33
Issue number6
DOIs
Publication statusPublished - Feb 14 2017
Externally publishedYes

Fingerprint

Photosystem II Protein Complex
Photocurrents
Gold
photocurrents
gold
Electrodes
electrodes
Platinum
platinum
Nanoparticles
nanoparticles
Irradiation
wire
Wire
irradiation
quinones
disulfides
conjugation
Absorption spectra
absorption spectra

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Miyachi, M., Ikehira, S., Nishiori, D., Yamanoi, Y., Yamada, M., Iwai, M., ... Nishihara, H. (2017). Photocurrent Generation of Reconstituted Photosystem II on a Self-Assembled Gold Film. Langmuir, 33(6), 1351-1358. https://doi.org/10.1021/acs.langmuir.6b03499

Photocurrent Generation of Reconstituted Photosystem II on a Self-Assembled Gold Film. / Miyachi, Mariko; Ikehira, Shu; Nishiori, Daiki; Yamanoi, Yoshinori; Yamada, Masato; Iwai, Masako; Tomo, Tatsuya; Allakhverdiev, Suleyman; Nishihara, Hiroshi.

In: Langmuir, Vol. 33, No. 6, 14.02.2017, p. 1351-1358.

Research output: Contribution to journalArticle

Miyachi, M, Ikehira, S, Nishiori, D, Yamanoi, Y, Yamada, M, Iwai, M, Tomo, T, Allakhverdiev, S & Nishihara, H 2017, 'Photocurrent Generation of Reconstituted Photosystem II on a Self-Assembled Gold Film', Langmuir, vol. 33, no. 6, pp. 1351-1358. https://doi.org/10.1021/acs.langmuir.6b03499
Miyachi M, Ikehira S, Nishiori D, Yamanoi Y, Yamada M, Iwai M et al. Photocurrent Generation of Reconstituted Photosystem II on a Self-Assembled Gold Film. Langmuir. 2017 Feb 14;33(6):1351-1358. https://doi.org/10.1021/acs.langmuir.6b03499
Miyachi, Mariko ; Ikehira, Shu ; Nishiori, Daiki ; Yamanoi, Yoshinori ; Yamada, Masato ; Iwai, Masako ; Tomo, Tatsuya ; Allakhverdiev, Suleyman ; Nishihara, Hiroshi. / Photocurrent Generation of Reconstituted Photosystem II on a Self-Assembled Gold Film. In: Langmuir. 2017 ; Vol. 33, No. 6. pp. 1351-1358.
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