Photochemical activity and the structure of chloroplasts in Arabidopsis thaliana L. mutants deficient in phytochrome A and B

Vladimir D. Kreslavski, Anatoly A. Kosobryukhov, Franz Josef Schmitt, Galina A. Semenova, Galina N. Shirshikova, Aleksandra Yu Khudyakova, Suleyman Allakhverdiev

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The reduced content of photoreceptors, such as phytochromes, can decrease the efficiency of photosynthesis and activity of the photosystem II (PSII). For the confirmation of this hypothesis, the effect of deficiency in both phytochromes (Phy) A and B (double mutant, DM) in 7–27-day-old Arabidopsis thaliana plants on the photosynthetic activity was studied in absence and presence of UV-A radiation as a stress factor. The DM with reduced content of apoproteins of PhyA and PhyB and wild type (WT) plants with were grown in white and red light (WL and RL, respectively) of high (130 μmol quanta m−2 s−1) and low (40 μmol quanta m−2 s−1) intensity. For DM and WT grown in WL, no notable difference in the photochemical activity of PSII was observed. However, the resistance of the photosynthetic apparatus (PA) to UV-A and the rate of photosynthesis under light saturation were lower in the DM compared to those in the WT. Growth in RL, when the photoreceptors of blue light—cryptochromes—are inactive, resulted in the significant decrease of the photochemical activity of PSII in DM compared to that in WT including amounts of QB-non-reducing complexes of PSII and noticeable enhancement of thermal dissipation of absorbed light energy. In addition, marked distortion of the thylakoid membrane structure was observed for DM grown in RL. It is suggested that not only PhyA and PhyB but also cryptochromes are necessary for normal functioning of the PA and formation of the mechanisms of its resistance to UV-radiation.

Original languageEnglish
Pages (from-to)1283-1293
Number of pages11
JournalProtoplasma
Volume254
Issue number3
DOIs
Publication statusPublished - May 1 2017
Externally publishedYes

Fingerprint

Phytochrome B
Phytochrome A
Photosystem II Protein Complex
Chloroplasts
phytochrome
Arabidopsis
Arabidopsis thaliana
chloroplasts
mutants
photosystem II
Photosynthesis
Light
Cryptochromes
Radiation
Phytochrome
photoreceptors
Thylakoids
Apoproteins
photosynthesis
apoproteins

Keywords

  • Arabidopsis thaliana L
  • Photosynthetic apparatus
  • Photosystem II
  • Phytochromes A and B
  • Stress resistance
  • UV-radiation

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Kreslavski, V. D., Kosobryukhov, A. A., Schmitt, F. J., Semenova, G. A., Shirshikova, G. N., Khudyakova, A. Y., & Allakhverdiev, S. (2017). Photochemical activity and the structure of chloroplasts in Arabidopsis thaliana L. mutants deficient in phytochrome A and B. Protoplasma, 254(3), 1283-1293. https://doi.org/10.1007/s00709-016-1020-9

Photochemical activity and the structure of chloroplasts in Arabidopsis thaliana L. mutants deficient in phytochrome A and B. / Kreslavski, Vladimir D.; Kosobryukhov, Anatoly A.; Schmitt, Franz Josef; Semenova, Galina A.; Shirshikova, Galina N.; Khudyakova, Aleksandra Yu; Allakhverdiev, Suleyman.

In: Protoplasma, Vol. 254, No. 3, 01.05.2017, p. 1283-1293.

Research output: Contribution to journalArticle

Kreslavski, VD, Kosobryukhov, AA, Schmitt, FJ, Semenova, GA, Shirshikova, GN, Khudyakova, AY & Allakhverdiev, S 2017, 'Photochemical activity and the structure of chloroplasts in Arabidopsis thaliana L. mutants deficient in phytochrome A and B', Protoplasma, vol. 254, no. 3, pp. 1283-1293. https://doi.org/10.1007/s00709-016-1020-9
Kreslavski VD, Kosobryukhov AA, Schmitt FJ, Semenova GA, Shirshikova GN, Khudyakova AY et al. Photochemical activity and the structure of chloroplasts in Arabidopsis thaliana L. mutants deficient in phytochrome A and B. Protoplasma. 2017 May 1;254(3):1283-1293. https://doi.org/10.1007/s00709-016-1020-9
Kreslavski, Vladimir D. ; Kosobryukhov, Anatoly A. ; Schmitt, Franz Josef ; Semenova, Galina A. ; Shirshikova, Galina N. ; Khudyakova, Aleksandra Yu ; Allakhverdiev, Suleyman. / Photochemical activity and the structure of chloroplasts in Arabidopsis thaliana L. mutants deficient in phytochrome A and B. In: Protoplasma. 2017 ; Vol. 254, No. 3. pp. 1283-1293.
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