Photoaffinity labeling of the phylloquinone-binding polypeptides by 2-azidoanthraquinone in photosystem I particles

Masayo Iwaki, Masaaki Takahashi, Keizo Shimada, Yuichiro Takahashi, Shigeru Itoh

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    A photoaffinity label, 2-azido-9,10-anthraquinone, binds at the quinone-binding (Qφ) site with high affinity and can substitute for the secondary acceptor, phylloquinone, in photosystem I reaction center of spinach. Phylloquinone-depleted photosystem I particles reconstituted with azido-[3H]anthraquinone were illuminated with UV light and analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis. The large core polypeptides (psaA and/or psaB) were selectively labeled. The labeling was competitively inhibited in the presence of anthraquinone. These results indicate that the Qφ site is located on psaA or psaB polypeptides.

    Original languageEnglish
    Pages (from-to)27-30
    Number of pages4
    JournalFEBS Letters
    Volume312
    Issue number1
    DOIs
    Publication statusPublished - Nov 2 1992

    Keywords

    • Electron transfer
    • Photoaffinity label
    • Photosystem I
    • Phylloquinone (vitamin K)
    • Reaction center

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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