Abstract
Blepharismin is an endogenous photosensitizing pigment found in the protozoan Blepharisma. This pigment inhibited the generation of Superoxide anion (O2-) in neutrophils not only via a diacylglycerol-induced protein kinase C (PKC)-dependent reaction but also by an arachidonate-induced PKC-independent reaction. The inhibition was light and concentration dependent for both reactions. Light-activated inhibition was strong at wavelengths between 520 and 570nm but not above 610nm. PKC activity in neutrophils and from rat brain was inhibited by blepharismin in a light- and concentration dependent manner. Moreover, arachidonate-activated NADPH oxidase activity in a cell-free system was also inhibited by the pigment in a light- and concentration-dependent manner. These results suggest that blepharismin inhibits NADPH oxidase activation through the non-specific inhibition of various membrane-bound enzymes and that this inhibition may also be correlated with that of PKC.
| Original language | English |
|---|---|
| Pages (from-to) | 529-536 |
| Number of pages | 8 |
| Journal | Biochemical Pharmacology |
| Volume | 49 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Feb 14 1995 |
| Externally published | Yes |
Fingerprint
Keywords
- active oxygen generation
- enzyme inhibition
- fluorescence of pigment
- NADPH-oxida se
- perylenequinones
- tyrosine kinase
ASJC Scopus subject areas
- Biochemistry
- Pharmacology
Cite this
Photoactivated inhibition of superoxide generation and protein kinase C activity in neutrophils by blepharismin, a protozoan photodynamically active pigment. / Watanabe, Yoshiya; E-ige, Keisuke; Kobuchi, Hirotsugu; Kato, Yoji; Matsuoka, Tatsuomi; Utsumi, Toshihiko; Yoshioka, Tamotsu; Horton, Alan A.; Utsumi, Kozo.
In: Biochemical Pharmacology, Vol. 49, No. 4, 14.02.1995, p. 529-536.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Photoactivated inhibition of superoxide generation and protein kinase C activity in neutrophils by blepharismin, a protozoan photodynamically active pigment
AU - Watanabe, Yoshiya
AU - E-ige, Keisuke
AU - Kobuchi, Hirotsugu
AU - Kato, Yoji
AU - Matsuoka, Tatsuomi
AU - Utsumi, Toshihiko
AU - Yoshioka, Tamotsu
AU - Horton, Alan A.
AU - Utsumi, Kozo
PY - 1995/2/14
Y1 - 1995/2/14
N2 - Blepharismin is an endogenous photosensitizing pigment found in the protozoan Blepharisma. This pigment inhibited the generation of Superoxide anion (O2-) in neutrophils not only via a diacylglycerol-induced protein kinase C (PKC)-dependent reaction but also by an arachidonate-induced PKC-independent reaction. The inhibition was light and concentration dependent for both reactions. Light-activated inhibition was strong at wavelengths between 520 and 570nm but not above 610nm. PKC activity in neutrophils and from rat brain was inhibited by blepharismin in a light- and concentration dependent manner. Moreover, arachidonate-activated NADPH oxidase activity in a cell-free system was also inhibited by the pigment in a light- and concentration-dependent manner. These results suggest that blepharismin inhibits NADPH oxidase activation through the non-specific inhibition of various membrane-bound enzymes and that this inhibition may also be correlated with that of PKC.
AB - Blepharismin is an endogenous photosensitizing pigment found in the protozoan Blepharisma. This pigment inhibited the generation of Superoxide anion (O2-) in neutrophils not only via a diacylglycerol-induced protein kinase C (PKC)-dependent reaction but also by an arachidonate-induced PKC-independent reaction. The inhibition was light and concentration dependent for both reactions. Light-activated inhibition was strong at wavelengths between 520 and 570nm but not above 610nm. PKC activity in neutrophils and from rat brain was inhibited by blepharismin in a light- and concentration dependent manner. Moreover, arachidonate-activated NADPH oxidase activity in a cell-free system was also inhibited by the pigment in a light- and concentration-dependent manner. These results suggest that blepharismin inhibits NADPH oxidase activation through the non-specific inhibition of various membrane-bound enzymes and that this inhibition may also be correlated with that of PKC.
KW - active oxygen generation
KW - enzyme inhibition
KW - fluorescence of pigment
KW - NADPH-oxida se
KW - perylenequinones
KW - tyrosine kinase
UR - http://www.scopus.com/inward/record.url?scp=0028815821&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028815821&partnerID=8YFLogxK
U2 - 10.1016/0006-2952(94)00409-F
DO - 10.1016/0006-2952(94)00409-F
M3 - Article
C2 - 7872958
AN - SCOPUS:0028815821
VL - 49
SP - 529
EP - 536
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
SN - 0006-2952
IS - 4
ER -