Photo inducible RNA interference using cell permeable protein carrier.

Tamaki Endoh; Masahiko Sisido; Takashi Ohtsuki

doi:10.1093/nass/nrm064

Photo inducible RNA interference using cell permeable protein carrier.

Tamaki Endoh, Masahiko Sisido, Takashi Ohtsuki

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

In this study, we constructed fluorescently labelled protein carrier constructed from cell-penetrating peptide (CPP) and RNA binding domain (RBD) for intracellular shRNA or siRNA delivery. The protein carrier specifically bound to an RNA cargo containing short extended sequence tag for protein binding, and internalized into CHO cells together with the RNA. Although the internalized protein/RNA complexes showed cytoplasmic punctuate distributions, they widely spread into cytosol by photo irradiation. Moreover, redistributed RNA induced gene silencing only within the photo irradiated area.

Original language	English
Pages (from-to)	127-128
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	51
DOIs	https://doi.org/10.1093/nass/nrm064
Publication status	Published - 2007

ASJC Scopus subject areas

Medicine(all)

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10.1093/nass/nrm064

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AU - Sisido, Masahiko

AU - Ohtsuki, Takashi

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PY - 2007

Y1 - 2007

N2 - In this study, we constructed fluorescently labelled protein carrier constructed from cell-penetrating peptide (CPP) and RNA binding domain (RBD) for intracellular shRNA or siRNA delivery. The protein carrier specifically bound to an RNA cargo containing short extended sequence tag for protein binding, and internalized into CHO cells together with the RNA. Although the internalized protein/RNA complexes showed cytoplasmic punctuate distributions, they widely spread into cytosol by photo irradiation. Moreover, redistributed RNA induced gene silencing only within the photo irradiated area.

AB - In this study, we constructed fluorescently labelled protein carrier constructed from cell-penetrating peptide (CPP) and RNA binding domain (RBD) for intracellular shRNA or siRNA delivery. The protein carrier specifically bound to an RNA cargo containing short extended sequence tag for protein binding, and internalized into CHO cells together with the RNA. Although the internalized protein/RNA complexes showed cytoplasmic punctuate distributions, they widely spread into cytosol by photo irradiation. Moreover, redistributed RNA induced gene silencing only within the photo irradiated area.

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Photo inducible RNA interference using cell permeable protein carrier.

Abstract

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