Photo inducible RNA interference using cell permeable protein carrier.

Tamaki Endoh, Masahiko Sisido, Takashi Ohtsuki

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In this study, we constructed fluorescently labelled protein carrier constructed from cell-penetrating peptide (CPP) and RNA binding domain (RBD) for intracellular shRNA or siRNA delivery. The protein carrier specifically bound to an RNA cargo containing short extended sequence tag for protein binding, and internalized into CHO cells together with the RNA. Although the internalized protein/RNA complexes showed cytoplasmic punctuate distributions, they widely spread into cytosol by photo irradiation. Moreover, redistributed RNA induced gene silencing only within the photo irradiated area.

Original languageEnglish
Pages (from-to)127-128
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number51
Publication statusPublished - 2007

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RNA Interference
Carrier Proteins
RNA
Small Interfering RNA
Cell-Penetrating Peptides
CHO Cells
Gene Silencing
Protein Binding
Cytosol
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Photo inducible RNA interference using cell permeable protein carrier. / Endoh, Tamaki; Sisido, Masahiko; Ohtsuki, Takashi.

In: Nucleic acids symposium series (2004), No. 51, 2007, p. 127-128.

Research output: Contribution to journalArticle

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