Photo-induced regulation of the chromatic adaptive gene expression by Anabaena sensory rhodopsin

Hiroki Irieda, Teppei Morita, Kimika Maki, Michio Homma, Hiroji Aiba, Yuki Sudo

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Rhodopsin molecules are photochemically reactive membrane-embedded proteins, with seven transmembrane α-helices, which bind the chromophore retinal (vitamin A aldehyde). They are roughly divided into two groups according to their basic functions: (i) ion transporters such as proton pumps, chloride pumps, and cation channels; and (ii) photo-sensors such as sensory rhodopsin from microbes and visual pigments from animals. Anabaena sensory rhodopsin (ASR), found in 2003 in the cyanobacterium Anabaena PCC7120, is categorized as a microbial sensory rhodopsin. To investigate the function of ASR in vivo, ASR and the promoter sequence of the pigment protein phycocyanin were co-introduced into Escherichia coli cells with the reporter gene crp. The result clearly showed that ASR functions as a repressor of the CRP protein expression and that this is fully inhibited by the light activation of ASR, suggesting that ASR would directly regulate the transcription of crp. The repression is also clearly inhibited by the truncation of the C-terminal region of ASR, or mutations on the C-terminal Arg residues, indicating the functional importance of the C-terminal region. Thus, our results demonstrate a novel function of rhodopsin molecules and raise the possibility that the membrane-spanning protein ASR could work as a transcriptional factor. In the future, the ASR activity could be utilized as a tool for arbitrary protein expression in vivo regulated by visible light.

Original languageEnglish
Pages (from-to)32485-32493
Number of pages9
JournalJournal of Biological Chemistry
Volume287
Issue number39
DOIs
Publication statusPublished - Sep 21 2012
Externally publishedYes

Fingerprint

Sensory Rhodopsins
Anabaena
Gene expression
Color
Gene Expression
Rhodopsin
Microbial Rhodopsins
Cation Transport Proteins
Membrane Proteins
Proteins
Phycocyanin
Retinaldehyde
Repressor Proteins
Light
Proton Pumps
Molecules
Retinal Pigments
Cyanobacteria
Transcription
Chromophores

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Photo-induced regulation of the chromatic adaptive gene expression by Anabaena sensory rhodopsin. / Irieda, Hiroki; Morita, Teppei; Maki, Kimika; Homma, Michio; Aiba, Hiroji; Sudo, Yuki.

In: Journal of Biological Chemistry, Vol. 287, No. 39, 21.09.2012, p. 32485-32493.

Research output: Contribution to journalArticle

Irieda, Hiroki ; Morita, Teppei ; Maki, Kimika ; Homma, Michio ; Aiba, Hiroji ; Sudo, Yuki. / Photo-induced regulation of the chromatic adaptive gene expression by Anabaena sensory rhodopsin. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 39. pp. 32485-32493.
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