Photo-induced proton transport of pharaonis phoborhodopsin (sensory rhodopsin II) is ceased by association with the transducer

Yuki Sudo, Masayuki Iwamoto, Kazumi Shimono, Masato Sumi, Naoki Kamo

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Phoborhodopsin (pR; also sensory rhodopsin II, sRII) is a retinoid protein in Halobacterium salinarum and works as a receptor of negative phototaxis. Pharaonis phoborhodopsin (ppR; also pharaonis sensory rhodopsin II, psRII) is a corresponding protein of Natronobacterium pharaonis. In bacterial membrane, ppR forms a complex with its transducer pHtrII, and this complex transmits the light signal to the sensory system in the cytoplasm. We expressed pHtrII-free ppR or ppR-pHtrII complex in H. salinarum Pho81/wr- cells. Flash-photolysis experiments showed no essential changes between pHtrII-free ppR and the complex. Using SnO2 electrode, which works as a sensitive pH electrode, and envelope membrane vesicles, we showed the photo-induced outward proton transport. This membranous proton transport was also shown using membrane vesicles from Escherichia coli in which ppR was functionally expressed. On the other hand, the proton transport was ceased when ppR formed a complex with pHtrII. Using membrane sheet, it was shown that the complex undergoes first proton uptake and then release during the photocycle, the same as pHtrII-free ppR, although the net proton transport ceases. Taking into consideration that the complex of sRII (pR) and its transducer undergoes extracellular proton circulation (J. Sasaki and J. L. Spudich, 1999, Biophys. J. 77:2145-2152), we inferred that association with pHtrII closes a cytoplasmic channel of ppR, which lead to the extracellular proton circulation.

Original languageEnglish
Pages (from-to)916-922
Number of pages7
JournalBiophysical Journal
Volume80
Issue number2
Publication statusPublished - 2001
Externally publishedYes

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Sensory Rhodopsins
Transducers
Protons
Halobacterium salinarum
Membranes
Natronobacterium
Electrodes
Photolysis
Retinoids
Cytoplasm
Proteins
Escherichia coli
Light

ASJC Scopus subject areas

  • Biophysics

Cite this

Photo-induced proton transport of pharaonis phoborhodopsin (sensory rhodopsin II) is ceased by association with the transducer. / Sudo, Yuki; Iwamoto, Masayuki; Shimono, Kazumi; Sumi, Masato; Kamo, Naoki.

In: Biophysical Journal, Vol. 80, No. 2, 2001, p. 916-922.

Research output: Contribution to journalArticle

Sudo, Yuki ; Iwamoto, Masayuki ; Shimono, Kazumi ; Sumi, Masato ; Kamo, Naoki. / Photo-induced proton transport of pharaonis phoborhodopsin (sensory rhodopsin II) is ceased by association with the transducer. In: Biophysical Journal. 2001 ; Vol. 80, No. 2. pp. 916-922.
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