Photo-acceleration of protein release from endosome in the protein transduction system

Masayuki Matsushita, Hirofumi Noguchi, Yun Fei Lu, Kazuhito Tomizawa, Hiroyuki Michiue, Sheng Tian Li, Kenzo Hirose, Susan Bonner-Weir, Hideki Matsui

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The protein transduction system has been employed for delivery of bioactive proteins into cells via an endocytotic mechanism. However, trapping of endocytosed proteins in the endosome may significantly attenuate biological actions in cells. The present investigation demonstrated that endosomal release of transduced protein could be artificially accelerated by exposure to fluorescent light. Exposure to light at 480 nm stimulated endosomal release of transduced FITC-11 arginine-protein transduction domain (11R-PTD), TAT-PTD and Antennapedia-PTD. Moreover, FITC-11R-p53 protein was released from endosomes following stimulation with light. These data suggest that photo-acceleration is a more efficient strategy in terms of the protein transduction system.

Original languageEnglish
Pages (from-to)221-226
Number of pages6
JournalFEBS Letters
Volume572
Issue number1-3
DOIs
Publication statusPublished - Aug 13 2004

Keywords

  • Endosomal release
  • FITC
  • Photo
  • Protein transduction
  • Protein transduction domain

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Matsushita, M., Noguchi, H., Lu, Y. F., Tomizawa, K., Michiue, H., Li, S. T., Hirose, K., Bonner-Weir, S., & Matsui, H. (2004). Photo-acceleration of protein release from endosome in the protein transduction system. FEBS Letters, 572(1-3), 221-226. https://doi.org/10.1016/j.febslet.2004.07.033