Phosphorylation of numb family proteins: Possible involvement of Ca 2+/calmodulin-dependent protein kinases

Hiroshi Tokumitsu, Naoya Hatano, Hiroyuki Inuzuka, Yuka Sueyoshi, Shigeyuki Yokokura, Tohru Ichimura, Naohito Nozaki, Ryoji Kobayashi

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

To search for the substrates of Ca2+/calmodulin-dependent protein kinase I (CaM-KI), we performed affinity chromatography purification using either the unphosphorylated or phosphorylated (at Thr177) GST-fused CaM-KI catalytic domain (residues 1-293, K49E) as the affinity ligand. Proteomic analysis was then carried out to identify the interacting proteins. In addition to the detection of two known CaM-KI substrates (CREB and synapsin I), we identified two Numb family proteins (Numb and Numbl) from rat tissues. These proteins were unphosphorylated and were bound only to the Thr 177-phosphorylated CaM-KI catalytic domain. This finding is consistent with the results demonstrating that Numb and Numbl were efficiently and stoichiometrically phosphorylated in vitro at equivalent Ser residues (Ser264 in Numb and Ser304 in Numbl) by activated CaM-KI and also by two other CaM-Ks (CaM-KII and CaM-KIV). Using anti-phospho-Numb/ Numbl antibody, we observed the phosphorylation of Numb family proteins in various rat tissue extracts, and we also detected the ionomycin-induced phosphorylation of endogenous Numb at Ser264 in COS-7 cells. The present results revealed that the Numb family proteins are phosphorylated in vivo as well as in vitro. Furthermore, we found that the recruitment of 14-3-3 proteins was the functional consequence of the phosphorylation of the Numb family proteins. Interaction of 14-3-3 protein with phosphorylated Numbl-blocked dephosphorylation of Ser304. Taken together, these results indicate that the Numb family proteins may be intracellular targets for CaM-Ks, and they may also be regulated by phosphorylation-dependent interaction with 14-3-3 protein.

Original languageEnglish
Pages (from-to)35108-35118
Number of pages11
JournalJournal of Biological Chemistry
Volume280
Issue number42
DOIs
Publication statusPublished - Oct 21 2005
Externally publishedYes

Fingerprint

Calcium-Calmodulin-Dependent Protein Kinase Type 1
Calcium-Calmodulin-Dependent Protein Kinases
Phosphorylation
14-3-3 Proteins
Proteins
Catalytic Domain
Rats
Synapsins
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Ionomycin
Tissue Extracts
COS Cells
Affinity chromatography
Affinity Chromatography
Proteomics
Substrates
Purification
Ligands
Tissue
Antibodies

ASJC Scopus subject areas

  • Biochemistry

Cite this

Phosphorylation of numb family proteins : Possible involvement of Ca 2+/calmodulin-dependent protein kinases. / Tokumitsu, Hiroshi; Hatano, Naoya; Inuzuka, Hiroyuki; Sueyoshi, Yuka; Yokokura, Shigeyuki; Ichimura, Tohru; Nozaki, Naohito; Kobayashi, Ryoji.

In: Journal of Biological Chemistry, Vol. 280, No. 42, 21.10.2005, p. 35108-35118.

Research output: Contribution to journalArticle

Tokumitsu, Hiroshi ; Hatano, Naoya ; Inuzuka, Hiroyuki ; Sueyoshi, Yuka ; Yokokura, Shigeyuki ; Ichimura, Tohru ; Nozaki, Naohito ; Kobayashi, Ryoji. / Phosphorylation of numb family proteins : Possible involvement of Ca 2+/calmodulin-dependent protein kinases. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 42. pp. 35108-35118.
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AU - Tokumitsu, Hiroshi

AU - Hatano, Naoya

AU - Inuzuka, Hiroyuki

AU - Sueyoshi, Yuka

AU - Yokokura, Shigeyuki

AU - Ichimura, Tohru

AU - Nozaki, Naohito

AU - Kobayashi, Ryoji

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AB - To search for the substrates of Ca2+/calmodulin-dependent protein kinase I (CaM-KI), we performed affinity chromatography purification using either the unphosphorylated or phosphorylated (at Thr177) GST-fused CaM-KI catalytic domain (residues 1-293, K49E) as the affinity ligand. Proteomic analysis was then carried out to identify the interacting proteins. In addition to the detection of two known CaM-KI substrates (CREB and synapsin I), we identified two Numb family proteins (Numb and Numbl) from rat tissues. These proteins were unphosphorylated and were bound only to the Thr 177-phosphorylated CaM-KI catalytic domain. This finding is consistent with the results demonstrating that Numb and Numbl were efficiently and stoichiometrically phosphorylated in vitro at equivalent Ser residues (Ser264 in Numb and Ser304 in Numbl) by activated CaM-KI and also by two other CaM-Ks (CaM-KII and CaM-KIV). Using anti-phospho-Numb/ Numbl antibody, we observed the phosphorylation of Numb family proteins in various rat tissue extracts, and we also detected the ionomycin-induced phosphorylation of endogenous Numb at Ser264 in COS-7 cells. The present results revealed that the Numb family proteins are phosphorylated in vivo as well as in vitro. Furthermore, we found that the recruitment of 14-3-3 proteins was the functional consequence of the phosphorylation of the Numb family proteins. Interaction of 14-3-3 protein with phosphorylated Numbl-blocked dephosphorylation of Ser304. Taken together, these results indicate that the Numb family proteins may be intracellular targets for CaM-Ks, and they may also be regulated by phosphorylation-dependent interaction with 14-3-3 protein.

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