Phosphorylation of inositol 1,4,5-triphosphate receptor 1 during in vitro maturation of porcine oocytes

Junya Ito, Tomoko Yoshida, Yasushi Kasai, Takuya Wakai, Jan B. Parys, Rafael A. Fissore, Naomi Kashiwazaki

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

During fertilization in mammalian species, a sperm-induced intracellular Ca2+ signal ([Ca2+]i) mediates both exit of meiosis and oocyte activation. Recently, we demonstrated in mouse oocytes that the phosphorylation levels of inositol 1,4,5 trisphosphate receptor type1 (IP3R1), the channel responsible for Ca2+ release and oscillations during fertilization, changed during maturation and fertilization. Therefore, we examined the expression and phosphorylation of IP3R1 during in vitro maturation of pig oocytes. Here, our present study shows that expression of IP3R1 protein did not change during maturation, although the phosphorylation status of the receptor, specifically at an MPM-2 epitope, did. We found that while at the beginning of maturation IP3R1 lacked MPM-2 immunoreactivity, it became MPM-2 reactive by 24 h and reached maximal reactivity by 36 h. Interestingly, the acquisition of MPM-2 reactivity coincided with the activation of p34cdc2 kinase and mitogen-activated protein kinase (MAPK), which are involved in meiotic progression. Following completion of maturation, inactivation of MAPK by U0126 did not affect IP3R1 phosphorylation, although inactivation of p34cdc2 kinase by roscovitine dramatically reduced IP3R1 phosphorylation. Neither inhibitor affected total expression of IP3R1. Altogether, our results show that IP3R1 undergoes dynamic phosphorylation during maturation and this might underlie the generation of oscillations at fertilization.

Original languageEnglish
Pages (from-to)34-41
Number of pages8
JournalAnimal Science Journal
Volume81
Issue number1
DOIs
Publication statusPublished - Feb 2010
Externally publishedYes

Fingerprint

In Vitro Oocyte Maturation Techniques
Inositol 1,4,5-Trisphosphate Receptors
phosphorylation
oocytes
Swine
Phosphorylation
Fertilization
receptors
swine
Mitogen-Activated Protein Kinases
mitogen-activated protein kinase
Oocytes
oscillation
inactivation
phosphotransferases (kinases)
Phosphotransferases
calcium
Meiosis
meiosis
epitopes

Keywords

  • Inositol triphosphate receptor
  • Kinase
  • Oocytes
  • Pig

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Phosphorylation of inositol 1,4,5-triphosphate receptor 1 during in vitro maturation of porcine oocytes. / Ito, Junya; Yoshida, Tomoko; Kasai, Yasushi; Wakai, Takuya; Parys, Jan B.; Fissore, Rafael A.; Kashiwazaki, Naomi.

In: Animal Science Journal, Vol. 81, No. 1, 02.2010, p. 34-41.

Research output: Contribution to journalArticle

Ito, Junya ; Yoshida, Tomoko ; Kasai, Yasushi ; Wakai, Takuya ; Parys, Jan B. ; Fissore, Rafael A. ; Kashiwazaki, Naomi. / Phosphorylation of inositol 1,4,5-triphosphate receptor 1 during in vitro maturation of porcine oocytes. In: Animal Science Journal. 2010 ; Vol. 81, No. 1. pp. 34-41.
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