Phosphorus starvation induces post-transcriptional CHS gene silencing in Petunia corolla

Munetaka Hosokawa, Takayoshi Yamauchi, Masayoshi Takahama, Mariko Goto, Sachiko Mikano, Yuki Yamaguchi, Yoshiyuki Tanaka, Sho Ohno, Sota Koeda, Motoaki Doi, Susumu Yazawa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Key message: The corolla ofPetunia'Magic Samba' exhibits unstable anthocyanin expression depending on its phosphorus content. Phosphorus deficiency enhanced post-transcriptional gene silencing ofchalcone synthase-Ain the corolla. Petunia (Petuniahybrida) 'Magic Samba' has unstable red-white bicolored corollas that respond to nutrient deficiency. We grew this cultivar hydroponically using solutions that lacked one or several nutrients to identify the specific nutrient related to anthocyanin expression in corolla. The white area of the corolla widened under phosphorus (P)-deficient conditions. When the P content of the corolla grown under P-deficient conditions dropped to 40 corollas until the plants died. Other elemental deficiencies had no clear effects on anthocyanin suppression in the corolla. After phosphate was resupplied to the P-deficient plants, anthocyanin was restored in the corollas. The expression of chalcone synthase-A(CHS-A) was suppressed in the white area that widened under P-suppressed conditions, whereas the expression of several other genes related to anthocyanin biosynthesis was enhanced more in the white area than in the red area. Reddish leaves and sepals developed under the P-deficient condition, which is a typical P-deficiency symptom. Two genes related to anthocyanin biosynthesis were enhanced in the reddish organs. Small interfering RNA analysis of CHS-A showed that the suppression resulted from post-transcriptional gene silencing (PTGS). Thus, it was hypothesized that the enhancement of anthocyanin biosynthetic gene expression due to P-deficiency triggered PTGS of CHS-A, which resulted in white corolla development.

Original languageEnglish
Pages (from-to)601-609
Number of pages9
JournalPlant Cell Reports
Volume32
Issue number5
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Petunia
RNA interference
corolla
starvation
anthocyanins
phosphorus
naringenin-chalcone synthase
biosynthesis
nutrients
nutrient deficiencies
small interfering RNA
calyx
signs and symptoms (plants)
genes
phosphates
gene expression
cultivars
leaves

Keywords

  • Anthocyanin
  • Chalcone synthase-A (CHS-A)
  • Petunia
  • Phosphorus deficiency
  • Post-transcriptional gene silencing (PTGS)

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Hosokawa, M., Yamauchi, T., Takahama, M., Goto, M., Mikano, S., Yamaguchi, Y., ... Yazawa, S. (2013). Phosphorus starvation induces post-transcriptional CHS gene silencing in Petunia corolla. Plant Cell Reports, 32(5), 601-609. https://doi.org/10.1007/s00299-013-1391-8

Phosphorus starvation induces post-transcriptional CHS gene silencing in Petunia corolla. / Hosokawa, Munetaka; Yamauchi, Takayoshi; Takahama, Masayoshi; Goto, Mariko; Mikano, Sachiko; Yamaguchi, Yuki; Tanaka, Yoshiyuki; Ohno, Sho; Koeda, Sota; Doi, Motoaki; Yazawa, Susumu.

In: Plant Cell Reports, Vol. 32, No. 5, 2013, p. 601-609.

Research output: Contribution to journalArticle

Hosokawa, M, Yamauchi, T, Takahama, M, Goto, M, Mikano, S, Yamaguchi, Y, Tanaka, Y, Ohno, S, Koeda, S, Doi, M & Yazawa, S 2013, 'Phosphorus starvation induces post-transcriptional CHS gene silencing in Petunia corolla', Plant Cell Reports, vol. 32, no. 5, pp. 601-609. https://doi.org/10.1007/s00299-013-1391-8
Hosokawa, Munetaka ; Yamauchi, Takayoshi ; Takahama, Masayoshi ; Goto, Mariko ; Mikano, Sachiko ; Yamaguchi, Yuki ; Tanaka, Yoshiyuki ; Ohno, Sho ; Koeda, Sota ; Doi, Motoaki ; Yazawa, Susumu. / Phosphorus starvation induces post-transcriptional CHS gene silencing in Petunia corolla. In: Plant Cell Reports. 2013 ; Vol. 32, No. 5. pp. 601-609.
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abstract = "Key message: The corolla ofPetunia'Magic Samba' exhibits unstable anthocyanin expression depending on its phosphorus content. Phosphorus deficiency enhanced post-transcriptional gene silencing ofchalcone synthase-Ain the corolla. Petunia (Petuniahybrida) 'Magic Samba' has unstable red-white bicolored corollas that respond to nutrient deficiency. We grew this cultivar hydroponically using solutions that lacked one or several nutrients to identify the specific nutrient related to anthocyanin expression in corolla. The white area of the corolla widened under phosphorus (P)-deficient conditions. When the P content of the corolla grown under P-deficient conditions dropped to 40 corollas until the plants died. Other elemental deficiencies had no clear effects on anthocyanin suppression in the corolla. After phosphate was resupplied to the P-deficient plants, anthocyanin was restored in the corollas. The expression of chalcone synthase-A(CHS-A) was suppressed in the white area that widened under P-suppressed conditions, whereas the expression of several other genes related to anthocyanin biosynthesis was enhanced more in the white area than in the red area. Reddish leaves and sepals developed under the P-deficient condition, which is a typical P-deficiency symptom. Two genes related to anthocyanin biosynthesis were enhanced in the reddish organs. Small interfering RNA analysis of CHS-A showed that the suppression resulted from post-transcriptional gene silencing (PTGS). Thus, it was hypothesized that the enhancement of anthocyanin biosynthetic gene expression due to P-deficiency triggered PTGS of CHS-A, which resulted in white corolla development.",
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AU - Goto, Mariko

AU - Mikano, Sachiko

AU - Yamaguchi, Yuki

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