Abstract
Plants grown in phosphorus-deprived solutions often exhibit disruption of water transport due to reduction in root hydraulic conductivity (Lpr). To uncover the relationship between root Lpr and water permeability coefficient (Pf) of plasma membrane and the role of aquaporins, we evaluated Pf of plasma membrane and also PIP-type aquaporin gene expression in tobacco (Nicotiana tabacum L.) plant roots after seven days P-deprivation. The results showed significant reduction in sap flow rate (Jv) and osmotic root hydraulic conductivity (Lpr-o) in P-deprived roots. These effects were reversed 24 h after P-resupplying. Interestingly, the Pf of root protoplasts was 57% lower in P-deprived plants compared with P-sufficient ones. The expression of NtPIP1;1 and NtPIP2;1 aquaporins did not change significantly in P-deprived plants compared with P-sufficient ones, but the copy number of NtAQP1 increased significantly in P-deprived plants. P-deprivation did not change Lpr-o significantly in antisense NtAQP1 plants. Taken together, these findings suggest that P-deprivation may play an important role in modulation of root hydraulic conductivity by affecting Pf in transcellular pathway of water flow across roots and aquaporins. Finally, we concluded that dominant water transport pathway under P-deprivation was transcellular one.
| Original language | English |
|---|---|
| Pages (from-to) | 54-61 |
| Number of pages | 8 |
| Journal | Russian Journal of Plant Physiology |
| Volume | 63 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2016 |
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Keywords
- aquaporins
- Nicotiana tabacum
- osmotic permeability coefficient
- osmotic root hydraulic conductivity
- phosphorus deprivation
- water relation
ASJC Scopus subject areas
- Plant Science
Cite this
Phosphorus deprivation effects on water relations of Nicotiana tabacum plant via reducing plasma membrane permeability. / Mahdieh, M.; Mostajeran, A.; Katsuhara, Maki.
In: Russian Journal of Plant Physiology, Vol. 63, No. 1, 01.01.2016, p. 54-61.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phosphorus deprivation effects on water relations of Nicotiana tabacum plant via reducing plasma membrane permeability
AU - Mahdieh, M.
AU - Mostajeran, A.
AU - Katsuhara, Maki
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Plants grown in phosphorus-deprived solutions often exhibit disruption of water transport due to reduction in root hydraulic conductivity (Lpr). To uncover the relationship between root Lpr and water permeability coefficient (Pf) of plasma membrane and the role of aquaporins, we evaluated Pf of plasma membrane and also PIP-type aquaporin gene expression in tobacco (Nicotiana tabacum L.) plant roots after seven days P-deprivation. The results showed significant reduction in sap flow rate (Jv) and osmotic root hydraulic conductivity (Lpr-o) in P-deprived roots. These effects were reversed 24 h after P-resupplying. Interestingly, the Pf of root protoplasts was 57% lower in P-deprived plants compared with P-sufficient ones. The expression of NtPIP1;1 and NtPIP2;1 aquaporins did not change significantly in P-deprived plants compared with P-sufficient ones, but the copy number of NtAQP1 increased significantly in P-deprived plants. P-deprivation did not change Lpr-o significantly in antisense NtAQP1 plants. Taken together, these findings suggest that P-deprivation may play an important role in modulation of root hydraulic conductivity by affecting Pf in transcellular pathway of water flow across roots and aquaporins. Finally, we concluded that dominant water transport pathway under P-deprivation was transcellular one.
AB - Plants grown in phosphorus-deprived solutions often exhibit disruption of water transport due to reduction in root hydraulic conductivity (Lpr). To uncover the relationship between root Lpr and water permeability coefficient (Pf) of plasma membrane and the role of aquaporins, we evaluated Pf of plasma membrane and also PIP-type aquaporin gene expression in tobacco (Nicotiana tabacum L.) plant roots after seven days P-deprivation. The results showed significant reduction in sap flow rate (Jv) and osmotic root hydraulic conductivity (Lpr-o) in P-deprived roots. These effects were reversed 24 h after P-resupplying. Interestingly, the Pf of root protoplasts was 57% lower in P-deprived plants compared with P-sufficient ones. The expression of NtPIP1;1 and NtPIP2;1 aquaporins did not change significantly in P-deprived plants compared with P-sufficient ones, but the copy number of NtAQP1 increased significantly in P-deprived plants. P-deprivation did not change Lpr-o significantly in antisense NtAQP1 plants. Taken together, these findings suggest that P-deprivation may play an important role in modulation of root hydraulic conductivity by affecting Pf in transcellular pathway of water flow across roots and aquaporins. Finally, we concluded that dominant water transport pathway under P-deprivation was transcellular one.
KW - aquaporins
KW - Nicotiana tabacum
KW - osmotic permeability coefficient
KW - osmotic root hydraulic conductivity
KW - phosphorus deprivation
KW - water relation
UR - http://www.scopus.com/inward/record.url?scp=84958259162&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84958259162&partnerID=8YFLogxK
U2 - 10.1134/S102144371601012X
DO - 10.1134/S102144371601012X
M3 - Article
VL - 63
SP - 54
EP - 61
JO - Russian Journal of Plant Physiology
JF - Russian Journal of Plant Physiology
SN - 1021-4437
IS - 1
ER -