Phosphatidylinositol 4,5-bisphosphate stimulates vesicle formation from liposomes by brain cytosol

Masahiro Kinuta, Hiroshi Yamada, Tadashi Abe, Masami Watanabe, Shun Ai Li, Akihiro Kamitani, Tatsuji Yasuda, Takashi Matsukawa, Hiromi Kumon, Kohji Takei

Research output: Contribution to journalArticle

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Abstract

As a step toward the elucidation of mechanisms in vesicle budding, a cell-free assay that measures cytosol-induced vesicle generation from liposomes was established. This assay then was used to explore the role of phosphoinositides in vesicle formation. Liposomes incubated with brain cytosol in the presence of ATP and GTP massively generated small vesicles, as assessed both quantitatively and qualitatively by a dynamic light-scattering assay. Both ATP and GTP were required. Vesicle formation was inhibited greatly by the immunodepletion of dynamin 1 from the cytosol, indicating a major contribution of this GTPase in this reaction and suggesting that it mimics endocytic vesicle fission. Increasing the concentration of L-α-phosphatidylinositol 4,5-bisphosphate [Ptdlns(4,5)P2] but not of L-α-phosphatidylinositol 4-monophosphate or L-α-phosphatidylinositol in the lipid membranes enhanced vesicle formation. Lipid analysis revealed rapid degradation of Ptdlns(4,5)P2 to L-α-phosphatidylinositol during the incubation with the reaction reaching a maximum within 5 sec, whereas vesicle formation proceeded with a longer time course. Ptdlns(4,5)P2 degradation was independent of vesicle formation and occurred also in the presence of guanosine 5′-O-(thiotriphosphate), where few vesicle formations occurred. These results suggest that Ptdlns(4,5)P2 plays a critical role in the early step of vesicle formation, possibly in the recruitment of coats and fission factors to membranes.

Original languageEnglish
Pages (from-to)2842-2847
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number5
DOIs
Publication statusPublished - Mar 5 2002

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Phosphatidylinositols
Liposomes
Cytosol
Brain
Guanosine Triphosphate
Dynamin I
Adenosine Triphosphate
Transport Vesicles
Guanosine
GTP Phosphohydrolases
Membrane Lipids
Lipids
Membranes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Phosphatidylinositol 4,5-bisphosphate stimulates vesicle formation from liposomes by brain cytosol. / Kinuta, Masahiro; Yamada, Hiroshi; Abe, Tadashi; Watanabe, Masami; Li, Shun Ai; Kamitani, Akihiro; Yasuda, Tatsuji; Matsukawa, Takashi; Kumon, Hiromi; Takei, Kohji.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 5, 05.03.2002, p. 2842-2847.

Research output: Contribution to journalArticle

Kinuta, Masahiro ; Yamada, Hiroshi ; Abe, Tadashi ; Watanabe, Masami ; Li, Shun Ai ; Kamitani, Akihiro ; Yasuda, Tatsuji ; Matsukawa, Takashi ; Kumon, Hiromi ; Takei, Kohji. / Phosphatidylinositol 4,5-bisphosphate stimulates vesicle formation from liposomes by brain cytosol. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 5. pp. 2842-2847.
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