Phosphatidic Acid Produced by Phospholipase D Promotes RNA Replication of a Plant RNA Virus

Kiwamu Hyoudou, Takako Taniguchi, Yuki Manabe, Masanori Kaido, Kazuyuki Mise, Tatsuya Sugawara, Hisaaki Taniguchi, Tetsuro Okuno

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Eukaryotic positive-strand RNA [(+)RNA] viruses are intracellular obligate parasites replicate using the membrane-bound replicase complexes that contain multiple viral and host components. To replicate, (+)RNA viruses exploit host resources and modify host metabolism and membrane organization. Phospholipase D (PLD) is a phosphatidylcholine- and phosphatidylethanolamine-hydrolyzing enzyme that catalyzes the production of phos phatidic acid (PA), a lipid second messenger that modulates diverse intracellular signaling in various organisms. PA is normally present in small amounts (less than 1% of total phospholipids), but rapidly and transiently accumulates in lipid bilayers in response to different environmental cues such as biotic and abiotic stresses in plants. However, the precise functions of PLD and PA remain unknown. Here, we report the roles of PLD and PA in genomic RNA replication of a plant (+)RNA virus, Red clover necrotic mosaic virus (RCNMV). We found that RCNMV RNA replication complexes formed in Nicotiana benthamiana contained PLDα and PLDβ. Gene-silencing and pharmacological inhibition approaches showed that PLDs and PLDs-derived PA are required for viral RNA replication. Consistent with this, exogenous application of PA enhanced viral RNA replication in plant cells and plant-derived cell-free extracts. We also found that a viral auxiliary replication protein bound to PA in vitro, and that the amount of PA increased in RCNMV-infected plant leaves. Together, our findings suggest that RCNMV hijacks host PA-producing enzymes to replicate.

Original languageEnglish
Article numbere1004909
JournalPLoS Pathogens
Volume11
Issue number5
DOIs
Publication statusPublished - May 1 2015

Fingerprint

Plant RNA
Plant Viruses
Phospholipase D
Phosphatidic Acids
RNA Viruses
Tombusviridae
Acids
Viral RNA
Plant Cells
RNA
DNA-like RNA
Viral Structures
Plant Leaves
Membranes
Lipid Bilayers
Second Messenger Systems
Gene Silencing
Enzymes
Virus Replication
Cell Extracts

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Hyoudou, K., Taniguchi, T., Manabe, Y., Kaido, M., Mise, K., Sugawara, T., ... Okuno, T. (2015). Phosphatidic Acid Produced by Phospholipase D Promotes RNA Replication of a Plant RNA Virus. PLoS Pathogens, 11(5), [e1004909]. https://doi.org/10.1371/journal.ppat.1004909

Phosphatidic Acid Produced by Phospholipase D Promotes RNA Replication of a Plant RNA Virus. / Hyoudou, Kiwamu; Taniguchi, Takako; Manabe, Yuki; Kaido, Masanori; Mise, Kazuyuki; Sugawara, Tatsuya; Taniguchi, Hisaaki; Okuno, Tetsuro.

In: PLoS Pathogens, Vol. 11, No. 5, e1004909, 01.05.2015.

Research output: Contribution to journalArticle

Hyoudou, K, Taniguchi, T, Manabe, Y, Kaido, M, Mise, K, Sugawara, T, Taniguchi, H & Okuno, T 2015, 'Phosphatidic Acid Produced by Phospholipase D Promotes RNA Replication of a Plant RNA Virus', PLoS Pathogens, vol. 11, no. 5, e1004909. https://doi.org/10.1371/journal.ppat.1004909
Hyoudou, Kiwamu ; Taniguchi, Takako ; Manabe, Yuki ; Kaido, Masanori ; Mise, Kazuyuki ; Sugawara, Tatsuya ; Taniguchi, Hisaaki ; Okuno, Tetsuro. / Phosphatidic Acid Produced by Phospholipase D Promotes RNA Replication of a Plant RNA Virus. In: PLoS Pathogens. 2015 ; Vol. 11, No. 5.
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