Phosphate or nitrate imbalance induces stronger molecular responses than combined nutrient deprivation in roots and leaves of chickpea plants

Maryam Nasr Esfahani, Komaki Inoue, Kien Huu Nguyen, Ha Duc Chu, Yasuko Watanabe, Asaka Kanatani, David J. Burritt, Keiichi Mochida, Lam Son Phan Tran

Research output: Contribution to journalArticlepeer-review

Abstract

The negative effects of phosphate (Pi) and/or nitrate (NO3) fertilizers on the environment have raised an urgent need to develop crop varieties with higher Pi and/or nitrogen use efficiencies for cultivation in low-fertility soils. Achieving this goal depends upon research that focuses on the identification of genes involved in plant responses to Pi and/or NO3 starvation. Although plant responses to individual deficiency in either Pi (–Pi/+NO3) or NO3 (+Pi/–NO3) have been separately studied, our understanding of plant responses to combined Pi and NO3 deficiency (–Pi/–NO3) is still very limited. Using RNA-sequencing approach, transcriptome changes in the roots and leaves of chickpea cultivated under –Pi/+NO3, +Pi/–NO3 or –Pi/–NO3 conditions were investigated in a comparative manner. –Pi/–NO3 treatment displayed lesser effect on expression changes of genes related to Pi or NO3 transport, signalling networks, lipid remodelling, nitrogen and Pi scavenging/remobilization/recycling, carbon metabolism and hormone metabolism than –Pi/+NO3 or +Pi/–NO3 treatments. Therefore, the plant response to –Pi/–NO3 is not simply an additive result of plant responses to –Pi/+NO3 and +Pi/–NO3 treatments. Our results indicate that nutrient imbalance is a stronger stimulus for molecular reprogramming than an overall deficiency.

Original languageEnglish
Pages (from-to)574-597
Number of pages24
JournalPlant Cell and Environment
Volume44
Issue number2
DOIs
Publication statusPublished - Feb 2021

Keywords

  • Cicer arietinum
  • RNA-sequencing
  • nitrate starvation
  • nitrate starvation-responsive genes
  • phosphate starvation
  • phosphate starvation-responsive genes

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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