Abstract
The early event of thymocyte maturation has been analyzed using acute lymphoblastic leukemia (ALL) cells. A group of ALL cells whose cell surface phenotype was CD2 (SRBC receptor) negative and CD7 (T cell antigen) positive has been considered as precursor thymocyte ALL (pre-T-ALL). No rearrangements of the T cell receptor β-gene (TCR β) and γ-gene (TCR Γ) were found in three of four pre-T-ALL patients. Stimulation of these pre-T-ALL cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) induced only CD25 (Tac) antigen but no other T cell antigens. These findings suggest that the activation pathway of interleukin 2 (IL-2) receptor already exists in the most immature precursor thymocytes. Pre-T-ALL cells from the fourth patients showed the expression of CD3 antigen, and both TCR β and TCR γ rearrangement. TPA induced the differentiation of the more mature pre-T-ALL cells of this case in vitro, and not only CD25 (Tac) antigen but also CD4 and CD8 antigens appeared on the cell surface. The low affinity binding of 125I-IL-2 to TPA-stimulated leukemia cells was observed in the three cases of pre-T-ALL tested, and the addition of recombinant IL-2 to TPA-stimulated cells showed no effect on cell proliferation.
| Original language | English |
|---|---|
| Pages (from-to) | 1291-1296 |
| Number of pages | 6 |
| Journal | Blood |
| Volume | 70 |
| Issue number | 5 |
| Publication status | Published - 1987 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Hematology
Cite this
Phorbol ester induces interleukin-2 receptor on the cell surface of precursor thymocyte leukemia with no rearrangement of T cell receptor β and γ genes. / Morishima, Y.; Morishita, Y.; Adachi, K.; Tanimoto, M.; Ohno, R.; Saito, H.
In: Blood, Vol. 70, No. 5, 1987, p. 1291-1296.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phorbol ester induces interleukin-2 receptor on the cell surface of precursor thymocyte leukemia with no rearrangement of T cell receptor β and γ genes
AU - Morishima, Y.
AU - Morishita, Y.
AU - Adachi, K.
AU - Tanimoto, M.
AU - Ohno, R.
AU - Saito, H.
PY - 1987
Y1 - 1987
N2 - The early event of thymocyte maturation has been analyzed using acute lymphoblastic leukemia (ALL) cells. A group of ALL cells whose cell surface phenotype was CD2 (SRBC receptor) negative and CD7 (T cell antigen) positive has been considered as precursor thymocyte ALL (pre-T-ALL). No rearrangements of the T cell receptor β-gene (TCR β) and γ-gene (TCR Γ) were found in three of four pre-T-ALL patients. Stimulation of these pre-T-ALL cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) induced only CD25 (Tac) antigen but no other T cell antigens. These findings suggest that the activation pathway of interleukin 2 (IL-2) receptor already exists in the most immature precursor thymocytes. Pre-T-ALL cells from the fourth patients showed the expression of CD3 antigen, and both TCR β and TCR γ rearrangement. TPA induced the differentiation of the more mature pre-T-ALL cells of this case in vitro, and not only CD25 (Tac) antigen but also CD4 and CD8 antigens appeared on the cell surface. The low affinity binding of 125I-IL-2 to TPA-stimulated leukemia cells was observed in the three cases of pre-T-ALL tested, and the addition of recombinant IL-2 to TPA-stimulated cells showed no effect on cell proliferation.
AB - The early event of thymocyte maturation has been analyzed using acute lymphoblastic leukemia (ALL) cells. A group of ALL cells whose cell surface phenotype was CD2 (SRBC receptor) negative and CD7 (T cell antigen) positive has been considered as precursor thymocyte ALL (pre-T-ALL). No rearrangements of the T cell receptor β-gene (TCR β) and γ-gene (TCR Γ) were found in three of four pre-T-ALL patients. Stimulation of these pre-T-ALL cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) induced only CD25 (Tac) antigen but no other T cell antigens. These findings suggest that the activation pathway of interleukin 2 (IL-2) receptor already exists in the most immature precursor thymocytes. Pre-T-ALL cells from the fourth patients showed the expression of CD3 antigen, and both TCR β and TCR γ rearrangement. TPA induced the differentiation of the more mature pre-T-ALL cells of this case in vitro, and not only CD25 (Tac) antigen but also CD4 and CD8 antigens appeared on the cell surface. The low affinity binding of 125I-IL-2 to TPA-stimulated leukemia cells was observed in the three cases of pre-T-ALL tested, and the addition of recombinant IL-2 to TPA-stimulated cells showed no effect on cell proliferation.
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M3 - Article
VL - 70
SP - 1291
EP - 1296
JO - Blood
JF - Blood
SN - 0006-4971
IS - 5
ER -