Phorbol ester induces interleukin-2 receptor on the cell surface of precursor thymocyte leukemia with no rearrangement of T cell receptor β and γ genes

Y. Morishima, Y. Morishita, K. Adachi, M. Tanimoto, R. Ohno, H. Saito

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The early event of thymocyte maturation has been analyzed using acute lymphoblastic leukemia (ALL) cells. A group of ALL cells whose cell surface phenotype was CD2 (SRBC receptor) negative and CD7 (T cell antigen) positive has been considered as precursor thymocyte ALL (pre-T-ALL). No rearrangements of the T cell receptor β-gene (TCR β) and γ-gene (TCR Γ) were found in three of four pre-T-ALL patients. Stimulation of these pre-T-ALL cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) induced only CD25 (Tac) antigen but no other T cell antigens. These findings suggest that the activation pathway of interleukin 2 (IL-2) receptor already exists in the most immature precursor thymocytes. Pre-T-ALL cells from the fourth patients showed the expression of CD3 antigen, and both TCR β and TCR γ rearrangement. TPA induced the differentiation of the more mature pre-T-ALL cells of this case in vitro, and not only CD25 (Tac) antigen but also CD4 and CD8 antigens appeared on the cell surface. The low affinity binding of 125I-IL-2 to TPA-stimulated leukemia cells was observed in the three cases of pre-T-ALL tested, and the addition of recombinant IL-2 to TPA-stimulated cells showed no effect on cell proliferation.

Original languageEnglish
Pages (from-to)1291-1296
Number of pages6
JournalBlood
Volume70
Issue number5
Publication statusPublished - 1987
Externally publishedYes

Fingerprint

T-Cell Receptor Genes
Interleukin-2 Receptors
Phorbol Esters
Thymocytes
T-Cell Antigen Receptor
Leukemia
Genes
Acetates
Tetradecanoylphorbol Acetate
T-cells
Antigens
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Interleukin-2 Receptor alpha Subunit
Interleukin-2
Viral Tumor Antigens
CD3 Antigens
CD8 Antigens
CD4 Antigens
Cell proliferation
T-Lymphocyte Gene Rearrangement

ASJC Scopus subject areas

  • Hematology

Cite this

Morishima, Y., Morishita, Y., Adachi, K., Tanimoto, M., Ohno, R., & Saito, H. (1987). Phorbol ester induces interleukin-2 receptor on the cell surface of precursor thymocyte leukemia with no rearrangement of T cell receptor β and γ genes. Blood, 70(5), 1291-1296.

Phorbol ester induces interleukin-2 receptor on the cell surface of precursor thymocyte leukemia with no rearrangement of T cell receptor β and γ genes. / Morishima, Y.; Morishita, Y.; Adachi, K.; Tanimoto, M.; Ohno, R.; Saito, H.

In: Blood, Vol. 70, No. 5, 1987, p. 1291-1296.

Research output: Contribution to journalArticle

Morishima, Y, Morishita, Y, Adachi, K, Tanimoto, M, Ohno, R & Saito, H 1987, 'Phorbol ester induces interleukin-2 receptor on the cell surface of precursor thymocyte leukemia with no rearrangement of T cell receptor β and γ genes', Blood, vol. 70, no. 5, pp. 1291-1296.
Morishima, Y. ; Morishita, Y. ; Adachi, K. ; Tanimoto, M. ; Ohno, R. ; Saito, H. / Phorbol ester induces interleukin-2 receptor on the cell surface of precursor thymocyte leukemia with no rearrangement of T cell receptor β and γ genes. In: Blood. 1987 ; Vol. 70, No. 5. pp. 1291-1296.
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