PhoB Regulates the Survival of Bacteroides fragilis in Peritoneal Abscesses

Shin Wakimoto, Haruyuki Nakayama-Imaohji, Minoru Ichimura, Hidetoshi Morita, Hideki Hirakawa, Tetsuya Hayashi, Koji Yasutomo, Tomomi Kuwahara

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In response to phosphate limitation, bacteria employ the Pho regulon, a specific regulatory network for phosphate acquisition. The two-component signal transduction system of PhoRB plays a crucial role in the induction of Pho regulon genes, leading to the adaptation to phosphate starvation. Herein, we identified the PhoRB system in Bacteroides fragilis, a commensal gut bacterium, and evaluated its role in gut colonization and survival in peritoneal abscesses. BF1575 and BF1576 encoded PhoR (sensor histidine kinase) and PhoB (response regulator) in the sequenced B. fragilis strain YCH46, respectively. Transcriptome analysis revealed that deletion of phoB affected the expression of 585 genes (more than 4-fold change) in B. fragilis, which included genes for stress response (chaperons and heat shock proteins), virulence (capsular polysaccharide biosynthesis) and phosphate metabolism. Deletion of phoB reduced the ability of the bacterium to persist in peritoneal abscesses induced by an intra-abdominal challenge of B. fragilis. Furthermore, PhoB was necessary for survival of this anaerobe in peritoneal abscesses but not for in vitro growth in rich media or in intestinal colonization. These results indicate that PhoB plays an important role in the survival of B. fragilis under stressful extraintestinal conditions.

Original languageEnglish
Article numbere53829
JournalPLoS One
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 15 2013
Externally publishedYes

Fingerprint

Bacteroides fragilis
abscess
Abscess
Phosphates
Bacteria
Genes
phosphates
Regulon
regulon
bacteria
Signal transduction
digestive system
Biosynthesis
Abdominal Abscess
histidine kinase
Heat-Shock Proteins
Histidine
Metabolism
anaerobes
Polysaccharides

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Wakimoto, S., Nakayama-Imaohji, H., Ichimura, M., Morita, H., Hirakawa, H., Hayashi, T., ... Kuwahara, T. (2013). PhoB Regulates the Survival of Bacteroides fragilis in Peritoneal Abscesses. PLoS One, 8(1), [e53829]. https://doi.org/10.1371/journal.pone.0053829

PhoB Regulates the Survival of Bacteroides fragilis in Peritoneal Abscesses. / Wakimoto, Shin; Nakayama-Imaohji, Haruyuki; Ichimura, Minoru; Morita, Hidetoshi; Hirakawa, Hideki; Hayashi, Tetsuya; Yasutomo, Koji; Kuwahara, Tomomi.

In: PLoS One, Vol. 8, No. 1, e53829, 15.01.2013.

Research output: Contribution to journalArticle

Wakimoto, S, Nakayama-Imaohji, H, Ichimura, M, Morita, H, Hirakawa, H, Hayashi, T, Yasutomo, K & Kuwahara, T 2013, 'PhoB Regulates the Survival of Bacteroides fragilis in Peritoneal Abscesses', PLoS One, vol. 8, no. 1, e53829. https://doi.org/10.1371/journal.pone.0053829
Wakimoto S, Nakayama-Imaohji H, Ichimura M, Morita H, Hirakawa H, Hayashi T et al. PhoB Regulates the Survival of Bacteroides fragilis in Peritoneal Abscesses. PLoS One. 2013 Jan 15;8(1). e53829. https://doi.org/10.1371/journal.pone.0053829
Wakimoto, Shin ; Nakayama-Imaohji, Haruyuki ; Ichimura, Minoru ; Morita, Hidetoshi ; Hirakawa, Hideki ; Hayashi, Tetsuya ; Yasutomo, Koji ; Kuwahara, Tomomi. / PhoB Regulates the Survival of Bacteroides fragilis in Peritoneal Abscesses. In: PLoS One. 2013 ; Vol. 8, No. 1.
@article{6e3f3c53f5394281b085993e659d7ae0,
title = "PhoB Regulates the Survival of Bacteroides fragilis in Peritoneal Abscesses",
abstract = "In response to phosphate limitation, bacteria employ the Pho regulon, a specific regulatory network for phosphate acquisition. The two-component signal transduction system of PhoRB plays a crucial role in the induction of Pho regulon genes, leading to the adaptation to phosphate starvation. Herein, we identified the PhoRB system in Bacteroides fragilis, a commensal gut bacterium, and evaluated its role in gut colonization and survival in peritoneal abscesses. BF1575 and BF1576 encoded PhoR (sensor histidine kinase) and PhoB (response regulator) in the sequenced B. fragilis strain YCH46, respectively. Transcriptome analysis revealed that deletion of phoB affected the expression of 585 genes (more than 4-fold change) in B. fragilis, which included genes for stress response (chaperons and heat shock proteins), virulence (capsular polysaccharide biosynthesis) and phosphate metabolism. Deletion of phoB reduced the ability of the bacterium to persist in peritoneal abscesses induced by an intra-abdominal challenge of B. fragilis. Furthermore, PhoB was necessary for survival of this anaerobe in peritoneal abscesses but not for in vitro growth in rich media or in intestinal colonization. These results indicate that PhoB plays an important role in the survival of B. fragilis under stressful extraintestinal conditions.",
author = "Shin Wakimoto and Haruyuki Nakayama-Imaohji and Minoru Ichimura and Hidetoshi Morita and Hideki Hirakawa and Tetsuya Hayashi and Koji Yasutomo and Tomomi Kuwahara",
year = "2013",
month = "1",
day = "15",
doi = "10.1371/journal.pone.0053829",
language = "English",
volume = "8",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "1",

}

TY - JOUR

T1 - PhoB Regulates the Survival of Bacteroides fragilis in Peritoneal Abscesses

AU - Wakimoto, Shin

AU - Nakayama-Imaohji, Haruyuki

AU - Ichimura, Minoru

AU - Morita, Hidetoshi

AU - Hirakawa, Hideki

AU - Hayashi, Tetsuya

AU - Yasutomo, Koji

AU - Kuwahara, Tomomi

PY - 2013/1/15

Y1 - 2013/1/15

N2 - In response to phosphate limitation, bacteria employ the Pho regulon, a specific regulatory network for phosphate acquisition. The two-component signal transduction system of PhoRB plays a crucial role in the induction of Pho regulon genes, leading to the adaptation to phosphate starvation. Herein, we identified the PhoRB system in Bacteroides fragilis, a commensal gut bacterium, and evaluated its role in gut colonization and survival in peritoneal abscesses. BF1575 and BF1576 encoded PhoR (sensor histidine kinase) and PhoB (response regulator) in the sequenced B. fragilis strain YCH46, respectively. Transcriptome analysis revealed that deletion of phoB affected the expression of 585 genes (more than 4-fold change) in B. fragilis, which included genes for stress response (chaperons and heat shock proteins), virulence (capsular polysaccharide biosynthesis) and phosphate metabolism. Deletion of phoB reduced the ability of the bacterium to persist in peritoneal abscesses induced by an intra-abdominal challenge of B. fragilis. Furthermore, PhoB was necessary for survival of this anaerobe in peritoneal abscesses but not for in vitro growth in rich media or in intestinal colonization. These results indicate that PhoB plays an important role in the survival of B. fragilis under stressful extraintestinal conditions.

AB - In response to phosphate limitation, bacteria employ the Pho regulon, a specific regulatory network for phosphate acquisition. The two-component signal transduction system of PhoRB plays a crucial role in the induction of Pho regulon genes, leading to the adaptation to phosphate starvation. Herein, we identified the PhoRB system in Bacteroides fragilis, a commensal gut bacterium, and evaluated its role in gut colonization and survival in peritoneal abscesses. BF1575 and BF1576 encoded PhoR (sensor histidine kinase) and PhoB (response regulator) in the sequenced B. fragilis strain YCH46, respectively. Transcriptome analysis revealed that deletion of phoB affected the expression of 585 genes (more than 4-fold change) in B. fragilis, which included genes for stress response (chaperons and heat shock proteins), virulence (capsular polysaccharide biosynthesis) and phosphate metabolism. Deletion of phoB reduced the ability of the bacterium to persist in peritoneal abscesses induced by an intra-abdominal challenge of B. fragilis. Furthermore, PhoB was necessary for survival of this anaerobe in peritoneal abscesses but not for in vitro growth in rich media or in intestinal colonization. These results indicate that PhoB plays an important role in the survival of B. fragilis under stressful extraintestinal conditions.

UR - http://www.scopus.com/inward/record.url?scp=84872411415&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872411415&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0053829

DO - 10.1371/journal.pone.0053829

M3 - Article

C2 - 23342014

AN - SCOPUS:84872411415

VL - 8

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 1

M1 - e53829

ER -