TY - JOUR
T1 - Phlorotannin Extracts from Ascophyllum nodosum Inhibited Proteases Activities and Structural Changes from Apostichopus japonicus
AU - Ming, Yu
AU - Wang, Yingzhen
AU - Xie, Yuqianqian
AU - Sun, Chenghang
AU - Dong, Xiufang
AU - Nakamura, Yoshimasa
AU - Chen, Xing
AU - Dong, Xiuping
AU - Qi, Hang
N1 - Funding Information:
This work was fnancially supported by The National Natural Science Foundation of China (No.31972143), Key Science and Technology Program of Liaoning Province (2020JH1/10200001), and Natural Science Foundation of Shandong Province (ZR2021QC168).
Publisher Copyright:
© 2022 The authors.
PY - 2022/10/21
Y1 - 2022/10/21
N2 - Fresh Apostichopus japonicas (A. japonicas) are easy to autolyze through the actions of endogenous proteases under certain circumstances, further inducing a decline in A. japonicas product quality and business value. To solve this serious problem in the A. japonicas industry, we investigated the inhibition of phlorotannin extracts (PhTE) on the activities and structural changes of crude endogenous proteinase (CEP) from A. japonicas. The results of enzyme activity and SDS-PAGE indicated that PhTE could inhibit enzyme activity in a dose dependent manner. Analysis of multiple spectroscopic methods, scanning electron microscopy, and differential scanning calorimetry indicated that PhTE promoted the cross-linking and aggregation of CEP and increased the particle size of the enzyme, thus changing the structure of the enzyme protein and decreasing the thermostability of protease. In conclusion, PhTE might be an effective inhibitor on endogenous proteinase in A. japonicas, which opened up a new way to prevent the A. japonicas autolysis.
AB - Fresh Apostichopus japonicas (A. japonicas) are easy to autolyze through the actions of endogenous proteases under certain circumstances, further inducing a decline in A. japonicas product quality and business value. To solve this serious problem in the A. japonicas industry, we investigated the inhibition of phlorotannin extracts (PhTE) on the activities and structural changes of crude endogenous proteinase (CEP) from A. japonicas. The results of enzyme activity and SDS-PAGE indicated that PhTE could inhibit enzyme activity in a dose dependent manner. Analysis of multiple spectroscopic methods, scanning electron microscopy, and differential scanning calorimetry indicated that PhTE promoted the cross-linking and aggregation of CEP and increased the particle size of the enzyme, thus changing the structure of the enzyme protein and decreasing the thermostability of protease. In conclusion, PhTE might be an effective inhibitor on endogenous proteinase in A. japonicas, which opened up a new way to prevent the A. japonicas autolysis.
KW - Apostichopus japonicas (A. japonicas)
KW - endogenous proteases
KW - inhibitory activity
KW - phlorotannin extracts
KW - structural changes
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U2 - 10.1021/acsfoodscitech.2c00216
DO - 10.1021/acsfoodscitech.2c00216
M3 - Article
AN - SCOPUS:85139558996
SN - 2692-1944
VL - 2
SP - 1586
EP - 1596
JO - ACS Food Science and Technology
JF - ACS Food Science and Technology
IS - 10
ER -