Phenylpropanoids as a protectant of aluminum toxicity in cultured tobacco cells

Yoko Yamamoto, Akiko Hachiya, Hiroki Hamada, Hideaki Matsumoto

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Aluminum (Al) enhances ferrous ion [Fe(II)]-mediated peroxidation of lipids, which is lethal to normal tobacco cells, but not to phosphate (P(i))-starved cells (-P cells). We found that tobacco cells accumulated phenylpropanoid compounds including chlorogenic acid (CGA) and caffeic acid (CA) during P(i) starvation. The accumulation was inhibited by 2-aminoindan-2-phosphonic acid (AIP), a specific inhibitor of L-phenylalanine ammonia lyase (PAL). CGA, CA and also an extract containing the phenylpropanoid compounds from -P cells protected normal cells (+P cells) efficiently from both lipid peroxidation and the loss of viability caused by the combined application of Al and Fe(II), indicating that the phenylpropanoids acted as antioxidant molecules. -P cells exhibited approximately 25-fold higher specific activity of PAL than +P cells. The content of the phenylpropanoids and the activity of PAL were not affected by the combined treatment with Al and Fe(II) in either +P cells or -P cells. These results suggest that an increase in PAL activity during P(i) starvation enhances the accumulation of phenylpropanoids, and that the phenylpropanoids protect tobacco cells from cytotoxic lipid peroxidation caused by the combination of Al and Fe(II).

Original languageEnglish
Pages (from-to)950-957
Number of pages8
JournalPlant and Cell Physiology
Volume39
Issue number9
Publication statusPublished - Sep 1998

Fingerprint

phenylpropanoids
Aluminum
Tobacco
aluminum
Cultured Cells
tobacco
toxicity
Phenylalanine Ammonia-Lyase
phenylalanine ammonia-lyase
cells
Lipid Peroxidation
Chlorogenic Acid
lipid peroxidation
caffeic acid
chlorogenic acid
Starvation
starvation
phosphorous acid
Antioxidants
Phosphates

Keywords

  • Aluminum
  • Lipid peroxidation
  • Nicotiana tabacum
  • Phenylalanine ammonia lyase
  • Phenylpropanoid
  • Phosphate starvation

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Physiology

Cite this

Yamamoto, Y., Hachiya, A., Hamada, H., & Matsumoto, H. (1998). Phenylpropanoids as a protectant of aluminum toxicity in cultured tobacco cells. Plant and Cell Physiology, 39(9), 950-957.

Phenylpropanoids as a protectant of aluminum toxicity in cultured tobacco cells. / Yamamoto, Yoko; Hachiya, Akiko; Hamada, Hiroki; Matsumoto, Hideaki.

In: Plant and Cell Physiology, Vol. 39, No. 9, 09.1998, p. 950-957.

Research output: Contribution to journalArticle

Yamamoto, Y, Hachiya, A, Hamada, H & Matsumoto, H 1998, 'Phenylpropanoids as a protectant of aluminum toxicity in cultured tobacco cells', Plant and Cell Physiology, vol. 39, no. 9, pp. 950-957.
Yamamoto Y, Hachiya A, Hamada H, Matsumoto H. Phenylpropanoids as a protectant of aluminum toxicity in cultured tobacco cells. Plant and Cell Physiology. 1998 Sep;39(9):950-957.
Yamamoto, Yoko ; Hachiya, Akiko ; Hamada, Hiroki ; Matsumoto, Hideaki. / Phenylpropanoids as a protectant of aluminum toxicity in cultured tobacco cells. In: Plant and Cell Physiology. 1998 ; Vol. 39, No. 9. pp. 950-957.
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