Phenotyping of isogenic chlorophyll-less bread and durum wheat mutant lines in relation to photoprotection and photosynthetic capacity

Marek Zivcak, Marian Brestic, Lenka Botyanszka, Yang Er Chen, Suleyman Allakhverdiev

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In our experiments, we examined high light responses and photosynthetic capacity of chlorophyll-less isogenic mutant lines of hexaploid bread wheat (Triticum aestivum L.) and tetraploid durum wheat (Triticum durum L.) in comparison to parental lines representing the wild type (WT), in two growth phases and two environments. In young plants, we observed a typical yellow-green phenotype with low chlorophyll content, significantly lower CO2 assimilation rate, elevated chlorophyll a-to-b ratio and insufficient regulation of linear electron transport. In the mutants grown in a moderate light in the growth chamber, a typical “chlorina” phenotype almost disappeared or, at least, was significantly alleviated in later growth stages, including the values of CO2 assimilation and the majority of the measured parameters related to photoprotective responses. On the other hand, in the case of the mutant lines grown in direct sunlight and fluctuating environment, the chlorophyll-less phenotype was evident also in latter growth phases. The chlorophyll-less phenotype was more severe in the durum wheat mutant lines compared to the bread wheat. For example, the durum wheat mutant lines grown outdoors expressed lower flexibility of photoprotective responses, including lower non-photochemical quenching and low rate of cyclic electron flow compared to WT or bread wheat mutants. Based on the analyses, we have identified a set of parameters providing information on the specific photosynthetic traits typical for the chlorophyll-less phenotype. Thus, the proposed way of phenotyping may serve for efficient selection of mutant genotypes for future research or screening activities. As a general result, we observed that the decrease of the chlorophyll content due to mutation was always associated with improper regulation of linear electron transport and a limited ability to prevent over-reduction of PSI acceptor side, regardless of the genotype, environment, and growth stage. This can partly explain why the low chlorophyll mutants were not successful in the evolution of higher plants, despite the photosynthetic capacity observed is high enough and they are fully competitive with wild-type plants in non-fluctuating controlled environment.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalPhotosynthesis Research
DOIs
Publication statusAccepted/In press - Jul 17 2018
Externally publishedYes

Fingerprint

photostability
Bread
Chlorophyll
durum wheat
Triticum
chlorophyll
phenotype
mutants
wheat
Phenotype
Growth
Electron Transport
electron transfer
assimilation (physiology)
Genotype
developmental stages
Light
Controlled Environment
Tetraploidy
Triticum turgidum subsp. durum

Keywords

  • Bread wheat
  • Chlorina
  • Durum wheat
  • Phenotyping
  • Photoinhibition
  • Photosystem I

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

Cite this

Phenotyping of isogenic chlorophyll-less bread and durum wheat mutant lines in relation to photoprotection and photosynthetic capacity. / Zivcak, Marek; Brestic, Marian; Botyanszka, Lenka; Chen, Yang Er; Allakhverdiev, Suleyman.

In: Photosynthesis Research, 17.07.2018, p. 1-13.

Research output: Contribution to journalArticle

Zivcak, Marek ; Brestic, Marian ; Botyanszka, Lenka ; Chen, Yang Er ; Allakhverdiev, Suleyman. / Phenotyping of isogenic chlorophyll-less bread and durum wheat mutant lines in relation to photoprotection and photosynthetic capacity. In: Photosynthesis Research. 2018 ; pp. 1-13.
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