Phenotypic modulation of the mesangium reflected by contractile proteins in diabetes

Hirofumi Makino, Naoki Kashihara, Hitoshi Sugiyama, Koichiro Kanao, Takashi Sekikawa, Kazunori Okamoto, Yohei Maeshima, Zensuke Ota, Ryozo Nagai

Research output: Contribution to journalArticle

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Abstract

The phenotypic change of the mesangial cell is considered to play a pivotal role in the accumulation of extracellular matrix in diabetic nephropathy. This investigation was undertaken to evaluate the expression of the various isoforms of contractile proteins in the streptozocin (STZ)- induced diabetic rat kidney and in renal biopsy specimens from patients with diabetic nephropathy. Specific antibodies to myosin heavy chain isoforms (SM1, SM2, SMemb), caldesmon, and α-smooth muscle actin and cDNAs for SMemb were used. Increased expression of SMemb at the mRNA and protein levels was demonstrated at 1 week after STZ administration in the rat. Both levels were increased at 4 weeks. Mesangial staining of caldesmon was observed at 4 weeks and that of α-smooth muscle actin at 24 weeks. Immunohistochemical mesangial staining of the contractile proteins was pronounced in patients with diabetic nephropathy in contrast to the trace mesangial staining in normal control subjects. These results indicate that the phenotypic change in mesangial cells occurs in the early stages of diabetes and that several stages in phenotypic changes may exist. Expression of the contractile protein isoforms, especially SMemb, should serve as a new marker for the subsequent glomerular hypertrophy and sclerosis.

Original languageEnglish
Pages (from-to)488-495
Number of pages8
JournalDiabetes
Volume45
Issue number4
Publication statusPublished - Apr 1996

Fingerprint

Contractile Proteins
Diabetic Nephropathies
Calmodulin-Binding Proteins
Protein Isoforms
Mesangial Cells
Streptozocin
Staining and Labeling
Smooth Muscle
Actins
Kidney
Myosin Heavy Chains
Sclerosis
Hypertrophy
Extracellular Matrix
Complementary DNA
Biopsy
Messenger RNA
Antibodies
Proteins

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Makino, H., Kashihara, N., Sugiyama, H., Kanao, K., Sekikawa, T., Okamoto, K., ... Nagai, R. (1996). Phenotypic modulation of the mesangium reflected by contractile proteins in diabetes. Diabetes, 45(4), 488-495.

Phenotypic modulation of the mesangium reflected by contractile proteins in diabetes. / Makino, Hirofumi; Kashihara, Naoki; Sugiyama, Hitoshi; Kanao, Koichiro; Sekikawa, Takashi; Okamoto, Kazunori; Maeshima, Yohei; Ota, Zensuke; Nagai, Ryozo.

In: Diabetes, Vol. 45, No. 4, 04.1996, p. 488-495.

Research output: Contribution to journalArticle

Makino, H, Kashihara, N, Sugiyama, H, Kanao, K, Sekikawa, T, Okamoto, K, Maeshima, Y, Ota, Z & Nagai, R 1996, 'Phenotypic modulation of the mesangium reflected by contractile proteins in diabetes', Diabetes, vol. 45, no. 4, pp. 488-495.
Makino H, Kashihara N, Sugiyama H, Kanao K, Sekikawa T, Okamoto K et al. Phenotypic modulation of the mesangium reflected by contractile proteins in diabetes. Diabetes. 1996 Apr;45(4):488-495.
Makino, Hirofumi ; Kashihara, Naoki ; Sugiyama, Hitoshi ; Kanao, Koichiro ; Sekikawa, Takashi ; Okamoto, Kazunori ; Maeshima, Yohei ; Ota, Zensuke ; Nagai, Ryozo. / Phenotypic modulation of the mesangium reflected by contractile proteins in diabetes. In: Diabetes. 1996 ; Vol. 45, No. 4. pp. 488-495.
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