Phenotypic and genomic comparisons of highly vancomycin-resistant Staphylococcus aureus strains developed from multiple clinical MRSA strains by in vitro mutagenesis

Kenichi Ishii, Fumiaki Tabuchi, Miki Matsuo, Keita Tatsuno, Tomoaki Sato, Mitsuhiro Okazaki, Hiroshi Hamamoto, Yasuhiko Matsumoto, Chikara Kaito, Tetsuji Aoyagi, Keiichi Hiramatsu, Mitsuo Kaku, Kyoji Moriya, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The development of vancomycin (VCM) resistance in Staphylococcus aureus threatens global health. Studies of the VCM-resistance mechanism and alternative therapeutic strategies are urgently needed. We mutagenized S. aureus laboratory strains and methicillin-resistant S. aureus (MRSA) with ethyl methanesulfonate, and isolated mutants that exhibited high resistance to VCM (minimum inhibitory concentration=32μg/ml). These VCM-resistant strains were sensitive to linezolid and rifampicin, and partly to arbekacin and daptomycin. Beta-lactams had synergistic effects with VCM against these mutants. VCM-resistant strains exhibited a 2-fold increase in the cell wall thickness. Several genes were commonly mutated among the highly VCM-resistant mutants. These findings suggest that MRSA has a potential to develop high VCM resistance with cell wall thickening by the accumulation of mutations.

Original languageEnglish
Article number17092
JournalScientific reports
Volume5
DOIs
Publication statusPublished - Nov 25 2015
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Phenotypic and genomic comparisons of highly vancomycin-resistant Staphylococcus aureus strains developed from multiple clinical MRSA strains by in vitro mutagenesis'. Together they form a unique fingerprint.

  • Cite this

    Ishii, K., Tabuchi, F., Matsuo, M., Tatsuno, K., Sato, T., Okazaki, M., Hamamoto, H., Matsumoto, Y., Kaito, C., Aoyagi, T., Hiramatsu, K., Kaku, M., Moriya, K., & Sekimizu, K. (2015). Phenotypic and genomic comparisons of highly vancomycin-resistant Staphylococcus aureus strains developed from multiple clinical MRSA strains by in vitro mutagenesis. Scientific reports, 5, [17092]. https://doi.org/10.1038/srep17092