Abstract
Incidence of epidemic Vibrio cholerae serogroup O139 has declined in cholera endemic countries. However, sporadic cholera caused by V. cholerae O139 with notable genetic changes is still reported from many regions. In the present study, 42 V. cholerae O139 strains isolated from 2001 to 2006 in Delhi, India, were retrospectively analyzed to understand their phenotype and molecular characteristics. The majority of isolates were resistant to ampicillin, furazolidone and nalidixic acid. Though the integrative conjugative element was detected in all the O139 isolates, the 2004-2006 isolates remained susceptible to co-trimoxazole, chloramphenicol, and streptomycin. Cholera toxin genotype 1 was present in the majority of the O139 isolates while few had type 3 or a novel type 4. In the cholera toxin encoding gene (ctx) restriction fragment length polymorphism, the majority of the isolates harbored three copies of CTX element, of which one was truncated. In this study, the ctx was detected for the first time in the small chromosome of V. cholerae O139 and one isolate harbored 5 copies of CTX element, of which 3 were truncated. The ribotype BII pattern was found in most of the O139 isolates. Three V. cholerae O139 isolated in 2001 had a new ribotype BVIII. Pulsed-field gel electrophoresis analysis revealed clonal variation in 2001 isolates compared to the 2004-2006 isolates. Molecular changes in V. cholerae O139 have to be closely monitored as this information may help in understanding the changing genetic features of this pathogen in relation to the epidemiology of cholera.
| Original language | English |
|---|---|
| Article number | 1250 |
| Journal | Frontiers in Microbiology |
| Volume | 7 |
| Issue number | AUG |
| DOIs | |
| Publication status | Published - Aug 9 2016 |
| Externally published | Yes |
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Keywords
- CT genotype
- CTX prophage
- PFGE
- Ribotypes
- V. cholerae O139
ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)
Cite this
Phenotypic and genetic heterogeneity in Vibrio cholerae O139 isolated from cholera cases in Delhi, India during 2001-2006. / Ghosh, Raikamal; Sharma, Naresh C.; Halder, Kalpataru; Bhadra, Rupak K.; Chowdhury, Goutam; Pazhani, Gururaja P.; Shinoda, Sumio; Mukhopadhyay, Asish K.; Balakrish Nair, G.; Ramamurthy, Thadavarayan.
In: Frontiers in Microbiology, Vol. 7, No. AUG, 1250, 09.08.2016.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phenotypic and genetic heterogeneity in Vibrio cholerae O139 isolated from cholera cases in Delhi, India during 2001-2006
AU - Ghosh, Raikamal
AU - Sharma, Naresh C.
AU - Halder, Kalpataru
AU - Bhadra, Rupak K.
AU - Chowdhury, Goutam
AU - Pazhani, Gururaja P.
AU - Shinoda, Sumio
AU - Mukhopadhyay, Asish K.
AU - Balakrish Nair, G.
AU - Ramamurthy, Thadavarayan
PY - 2016/8/9
Y1 - 2016/8/9
N2 - Incidence of epidemic Vibrio cholerae serogroup O139 has declined in cholera endemic countries. However, sporadic cholera caused by V. cholerae O139 with notable genetic changes is still reported from many regions. In the present study, 42 V. cholerae O139 strains isolated from 2001 to 2006 in Delhi, India, were retrospectively analyzed to understand their phenotype and molecular characteristics. The majority of isolates were resistant to ampicillin, furazolidone and nalidixic acid. Though the integrative conjugative element was detected in all the O139 isolates, the 2004-2006 isolates remained susceptible to co-trimoxazole, chloramphenicol, and streptomycin. Cholera toxin genotype 1 was present in the majority of the O139 isolates while few had type 3 or a novel type 4. In the cholera toxin encoding gene (ctx) restriction fragment length polymorphism, the majority of the isolates harbored three copies of CTX element, of which one was truncated. In this study, the ctx was detected for the first time in the small chromosome of V. cholerae O139 and one isolate harbored 5 copies of CTX element, of which 3 were truncated. The ribotype BII pattern was found in most of the O139 isolates. Three V. cholerae O139 isolated in 2001 had a new ribotype BVIII. Pulsed-field gel electrophoresis analysis revealed clonal variation in 2001 isolates compared to the 2004-2006 isolates. Molecular changes in V. cholerae O139 have to be closely monitored as this information may help in understanding the changing genetic features of this pathogen in relation to the epidemiology of cholera.
AB - Incidence of epidemic Vibrio cholerae serogroup O139 has declined in cholera endemic countries. However, sporadic cholera caused by V. cholerae O139 with notable genetic changes is still reported from many regions. In the present study, 42 V. cholerae O139 strains isolated from 2001 to 2006 in Delhi, India, were retrospectively analyzed to understand their phenotype and molecular characteristics. The majority of isolates were resistant to ampicillin, furazolidone and nalidixic acid. Though the integrative conjugative element was detected in all the O139 isolates, the 2004-2006 isolates remained susceptible to co-trimoxazole, chloramphenicol, and streptomycin. Cholera toxin genotype 1 was present in the majority of the O139 isolates while few had type 3 or a novel type 4. In the cholera toxin encoding gene (ctx) restriction fragment length polymorphism, the majority of the isolates harbored three copies of CTX element, of which one was truncated. In this study, the ctx was detected for the first time in the small chromosome of V. cholerae O139 and one isolate harbored 5 copies of CTX element, of which 3 were truncated. The ribotype BII pattern was found in most of the O139 isolates. Three V. cholerae O139 isolated in 2001 had a new ribotype BVIII. Pulsed-field gel electrophoresis analysis revealed clonal variation in 2001 isolates compared to the 2004-2006 isolates. Molecular changes in V. cholerae O139 have to be closely monitored as this information may help in understanding the changing genetic features of this pathogen in relation to the epidemiology of cholera.
KW - CT genotype
KW - CTX prophage
KW - PFGE
KW - Ribotypes
KW - V. cholerae O139
UR - http://www.scopus.com/inward/record.url?scp=84989213209&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84989213209&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2016.01250
DO - 10.3389/fmicb.2016.01250
M3 - Article
VL - 7
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
SN - 1664-302X
IS - AUG
M1 - 1250
ER -