Phenotypic and functional characterization of T-cell lines generated from colonoscopic biopsy specimens in patients with ulcerative colitis

Kazuo Kusugami, Jun Ichi Haruta, Mayumi Ieda, Masataka Shinoda, Takafumi Ando, Atsuo Kuroiwa, Kenji Ina, Hitoshi Iokawa, Akira Ishihara, Susumu Sarai, Yuichiro Saito, Mitsune Tanimoto, Kimitomo Morise

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Intestinal T-cell lines were generated from lamina propria mononuclear cells isolated from colonoscopic biopsies in ulcerative colitis patients and controls. In both ulcerative colitis and controls, expanded cells were constituted largely by T-cell receptor αβ+, CD4+, CD45RA- (helper), and CD8+, CD11b- (cytotoxic) phenotypes. T-cell receptor Vβ gene usage was not significantly changed after cell expansion and no difference was observed between ulcerative colitis and controls. Ulcerative colitis cells, especially those derived from the patients with long-standing disease, showed significantly higher levels of cytotoxicity against the target cells, including those of colonic epithelial origin, and enhanced production of tumor necrosis factor-α and interferon-γ after short incubation with anti-CD3 antibody. Generation of T-cell lines from colonoscopic biopsy specimens may be useful for detailed functional characterization of locally infiltrating T cells in ulcerative colitis patients.

Original languageEnglish
Pages (from-to)198-210
Number of pages13
JournalDigestive Diseases and Sciences
Volume40
Issue number1
DOIs
Publication statusPublished - Jan 1 1995

Keywords

  • T-cell lines
  • T-cell receptor
  • colonoscopic biopsy specimens
  • cytokine
  • cytotoxicity
  • ulcerative colitis

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

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