Phenotypic analysis of monocyte-derived dendritic cells loaded with tumor antigen with heat-shock cognate protein-70

Shinichiro Ito, Yasuhiro Nagata, Seiya Susumu, Akira Yoneda, Mitsutoshi Matsuo, Katsuyuki Yui, Heiichiro Udono, Susumu Eguchi, Takashi Kanematsu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background/Aim: The cross-presentation system of tumor antigen by monocyte-derived dendritic cells (mo-DCs) has been observed under appropriate conditions. Both CD14- negative and CD1a-positive phenotypes were critical in our previous study. This study compared the phenotype of mo-DCs and identified the conditions that favored T helper-1 (Th1) cytokine production after stimulation with the hsc70 and NY-ESO- 1 p157-165 epitope fusion protein (hsc70/ESO p157- 165). Materials and Methods: The mo-DCs were induced from healthy donors. Their surface markers and cytokine production were examined after stimulation with hsc70/ESO p157-165. Results: CD1a+ and CD1a- mo-DCs were generated in half of the healthy donors. The concentration of fetal calf serum in the culture medium was critical for the induction of CD1a+ DCs, which were able to produce interleukin-12 (IL-12), but not IL-10. Neutralizing IL-6 and IL-6R antibodies affected the expression of CD1a. Conclusion: Anti IL-6 analogs may be effective adjuvants for the development of mo-DC-based cancer vaccine.

Original languageEnglish
Pages (from-to)4897-4904
Number of pages8
JournalAnticancer Research
Volume32
Issue number11
Publication statusPublished - Nov 2012

Fingerprint

HSC70 Heat-Shock Proteins
Neoplasm Antigens
Dendritic Cells
Monocytes
Interleukin-6
Cross-Priming
Cytokines
Phenotype
Cancer Vaccines
Interleukin-12
Interleukin-10
Culture Media
Epitopes
Antibodies
Serum

Keywords

  • Dendritic cell
  • Hsc-70
  • IL-6
  • NY-ESO-1
  • Phenotypic analysis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ito, S., Nagata, Y., Susumu, S., Yoneda, A., Matsuo, M., Yui, K., ... Kanematsu, T. (2012). Phenotypic analysis of monocyte-derived dendritic cells loaded with tumor antigen with heat-shock cognate protein-70. Anticancer Research, 32(11), 4897-4904.

Phenotypic analysis of monocyte-derived dendritic cells loaded with tumor antigen with heat-shock cognate protein-70. / Ito, Shinichiro; Nagata, Yasuhiro; Susumu, Seiya; Yoneda, Akira; Matsuo, Mitsutoshi; Yui, Katsuyuki; Udono, Heiichiro; Eguchi, Susumu; Kanematsu, Takashi.

In: Anticancer Research, Vol. 32, No. 11, 11.2012, p. 4897-4904.

Research output: Contribution to journalArticle

Ito, S, Nagata, Y, Susumu, S, Yoneda, A, Matsuo, M, Yui, K, Udono, H, Eguchi, S & Kanematsu, T 2012, 'Phenotypic analysis of monocyte-derived dendritic cells loaded with tumor antigen with heat-shock cognate protein-70', Anticancer Research, vol. 32, no. 11, pp. 4897-4904.
Ito S, Nagata Y, Susumu S, Yoneda A, Matsuo M, Yui K et al. Phenotypic analysis of monocyte-derived dendritic cells loaded with tumor antigen with heat-shock cognate protein-70. Anticancer Research. 2012 Nov;32(11):4897-4904.
Ito, Shinichiro ; Nagata, Yasuhiro ; Susumu, Seiya ; Yoneda, Akira ; Matsuo, Mitsutoshi ; Yui, Katsuyuki ; Udono, Heiichiro ; Eguchi, Susumu ; Kanematsu, Takashi. / Phenotypic analysis of monocyte-derived dendritic cells loaded with tumor antigen with heat-shock cognate protein-70. In: Anticancer Research. 2012 ; Vol. 32, No. 11. pp. 4897-4904.
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