Phenotypic analysis of monocyte-derived dendritic cells loaded with tumor antigen with heat-shock cognate protein-70

Shinichiro Ito, Yasuhiro Nagata, Seiya Susumu, Akira Yoneda, Mitsutoshi Matsuo, Katsuyuki Yui, Heiichiro Udono, Susumu Eguchi, Takashi Kanematsu

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2 Citations (Scopus)

Abstract

Background/Aim: The cross-presentation system of tumor antigen by monocyte-derived dendritic cells (mo-DCs) has been observed under appropriate conditions. Both CD14- negative and CD1a-positive phenotypes were critical in our previous study. This study compared the phenotype of mo-DCs and identified the conditions that favored T helper-1 (Th1) cytokine production after stimulation with the hsc70 and NY-ESO- 1 p157-165 epitope fusion protein (hsc70/ESO p157- 165). Materials and Methods: The mo-DCs were induced from healthy donors. Their surface markers and cytokine production were examined after stimulation with hsc70/ESO p157-165. Results: CD1a+ and CD1a- mo-DCs were generated in half of the healthy donors. The concentration of fetal calf serum in the culture medium was critical for the induction of CD1a+ DCs, which were able to produce interleukin-12 (IL-12), but not IL-10. Neutralizing IL-6 and IL-6R antibodies affected the expression of CD1a. Conclusion: Anti IL-6 analogs may be effective adjuvants for the development of mo-DC-based cancer vaccine.

Original languageEnglish
Pages (from-to)4897-4904
Number of pages8
JournalAnticancer research
Volume32
Issue number11
Publication statusPublished - Nov 2012

Keywords

  • Dendritic cell
  • Hsc-70
  • IL-6
  • NY-ESO-1
  • Phenotypic analysis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Ito, S., Nagata, Y., Susumu, S., Yoneda, A., Matsuo, M., Yui, K., Udono, H., Eguchi, S., & Kanematsu, T. (2012). Phenotypic analysis of monocyte-derived dendritic cells loaded with tumor antigen with heat-shock cognate protein-70. Anticancer research, 32(11), 4897-4904.