Phased A-tracts bind to the α subunit of RNA polymerase with increased affinity at low temperature

Seiichi Katayama, Osamu Matsushita, Eiji Tamai, Shigeru Miyata, Akinobu Okabe

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Previously we showed that the expression of a Clostridium perfringens phospholipase C gene (plc) is activated by promoter upstream phased A-tracts in a low temperature-dependent manner. In this paper we characterize the interaction between the α subunit of C. perfringens RNA polymerase and the phased A-tracts. Hydroxyl radical footprinting and fluorescence polarization assaying revealed that the α subunit binds to the minor grooves of the phased A-tracts through its C-terminal domain with increased affinity at low temperature. The result provides a molecular mechanism underlying the activation of the plc promoter by the phased A-tracts.

Original languageEnglish
Pages (from-to)235-238
Number of pages4
JournalFEBS Letters
Volume509
Issue number2
DOIs
Publication statusPublished - Dec 7 2001
Externally publishedYes

Keywords

  • Clostridium perfringens
  • Curved DNA
  • Gene expression
  • Phospholipase C
  • RNA polymerase
  • α Subunit

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Phased A-tracts bind to the α subunit of RNA polymerase with increased affinity at low temperature'. Together they form a unique fingerprint.

Cite this