Phased A-tracts bind to the α subunit of RNA polymerase with increased affinity at low temperature

Seiichi Katayama, Osamu Matsushita, Eiji Tamai, Shigeru Miyata, Akinobu Okabe

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Previously we showed that the expression of a Clostridium perfringens phospholipase C gene (plc) is activated by promoter upstream phased A-tracts in a low temperature-dependent manner. In this paper we characterize the interaction between the α subunit of C. perfringens RNA polymerase and the phased A-tracts. Hydroxyl radical footprinting and fluorescence polarization assaying revealed that the α subunit binds to the minor grooves of the phased A-tracts through its C-terminal domain with increased affinity at low temperature. The result provides a molecular mechanism underlying the activation of the plc promoter by the phased A-tracts.

Original languageEnglish
Pages (from-to)235-238
Number of pages4
JournalFEBS Letters
Volume509
Issue number2
DOIs
Publication statusPublished - Dec 7 2001
Externally publishedYes

Fingerprint

Clostridium perfringens
Type C Phospholipases
DNA-Directed RNA Polymerases
Genes
Clostridium
Temperature
Fluorescence Polarization
Hydroxyl Radical
Fluorescence
Chemical activation
Polarization

Keywords

  • α Subunit
  • Clostridium perfringens
  • Curved DNA
  • Gene expression
  • Phospholipase C
  • RNA polymerase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Phased A-tracts bind to the α subunit of RNA polymerase with increased affinity at low temperature. / Katayama, Seiichi; Matsushita, Osamu; Tamai, Eiji; Miyata, Shigeru; Okabe, Akinobu.

In: FEBS Letters, Vol. 509, No. 2, 07.12.2001, p. 235-238.

Research output: Contribution to journalArticle

Katayama, Seiichi ; Matsushita, Osamu ; Tamai, Eiji ; Miyata, Shigeru ; Okabe, Akinobu. / Phased A-tracts bind to the α subunit of RNA polymerase with increased affinity at low temperature. In: FEBS Letters. 2001 ; Vol. 509, No. 2. pp. 235-238.
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