Phase-shifting the fruit fly clock without cryptochrome

Christa Kistenpfennig, Jay Hirsh, Taishi Yoshii, Charlotte Helfrich-Förster

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The blue light photopigment cryptochrome (CRY) is thought to be the main circadian photoreceptor of Drosophila melanogaster. Nevertheless, entrainment to light-dark cycles is possible without functional CRY. Here, we monitored phase response curves of cry01 mutants and control flies to 1-hour 1000-lux light pulses. We found that cry01 mutants phase-shift their activity rhythm in the subjective early morning and late evening, although with reduced magnitude. This phase-shifting capability is sufficient for the slowed entrainment of the mutants, indicating that the eyes contribute to the clock's light sensitivity around dawn and dusk. With longer light pulses (3 hours and 6 hours), wild-type flies show greatly enhanced magnitude of phase shift, but CRY-less flies seem impaired in the ability to integrate duration of the light pulse in a wild-type manner: Only 6-hour light pulses at circadian time 21 significantly increased the magnitude of phase advances in cry01 mutants. At circadian time 15, the mutants exhibited phase advances instead of the expected delays. These complex results are discussed.

Original languageEnglish
Pages (from-to)117-125
Number of pages9
JournalJournal of Biological Rhythms
Volume27
Issue number2
DOIs
Publication statusPublished - Apr 2012

Fingerprint

Cryptochromes
Diptera
Fruit
Light
Photophobia
Photoperiod
Drosophila melanogaster

Keywords

  • cryptochrome
  • Drosophila melanogaster
  • light pulses
  • locomotor activity

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Phase-shifting the fruit fly clock without cryptochrome. / Kistenpfennig, Christa; Hirsh, Jay; Yoshii, Taishi; Helfrich-Förster, Charlotte.

In: Journal of Biological Rhythms, Vol. 27, No. 2, 04.2012, p. 117-125.

Research output: Contribution to journalArticle

Kistenpfennig, C, Hirsh, J, Yoshii, T & Helfrich-Förster, C 2012, 'Phase-shifting the fruit fly clock without cryptochrome', Journal of Biological Rhythms, vol. 27, no. 2, pp. 117-125. https://doi.org/10.1177/0748730411434390
Kistenpfennig, Christa ; Hirsh, Jay ; Yoshii, Taishi ; Helfrich-Förster, Charlotte. / Phase-shifting the fruit fly clock without cryptochrome. In: Journal of Biological Rhythms. 2012 ; Vol. 27, No. 2. pp. 117-125.
@article{1b2bede340fe4b64a6addbe26bd24d44,
title = "Phase-shifting the fruit fly clock without cryptochrome",
abstract = "The blue light photopigment cryptochrome (CRY) is thought to be the main circadian photoreceptor of Drosophila melanogaster. Nevertheless, entrainment to light-dark cycles is possible without functional CRY. Here, we monitored phase response curves of cry01 mutants and control flies to 1-hour 1000-lux light pulses. We found that cry01 mutants phase-shift their activity rhythm in the subjective early morning and late evening, although with reduced magnitude. This phase-shifting capability is sufficient for the slowed entrainment of the mutants, indicating that the eyes contribute to the clock's light sensitivity around dawn and dusk. With longer light pulses (3 hours and 6 hours), wild-type flies show greatly enhanced magnitude of phase shift, but CRY-less flies seem impaired in the ability to integrate duration of the light pulse in a wild-type manner: Only 6-hour light pulses at circadian time 21 significantly increased the magnitude of phase advances in cry01 mutants. At circadian time 15, the mutants exhibited phase advances instead of the expected delays. These complex results are discussed.",
keywords = "cryptochrome, Drosophila melanogaster, light pulses, locomotor activity",
author = "Christa Kistenpfennig and Jay Hirsh and Taishi Yoshii and Charlotte Helfrich-F{\"o}rster",
year = "2012",
month = "4",
doi = "10.1177/0748730411434390",
language = "English",
volume = "27",
pages = "117--125",
journal = "Journal of Biological Rhythms",
issn = "0748-7304",
publisher = "SAGE Publications Inc.",
number = "2",

}

TY - JOUR

T1 - Phase-shifting the fruit fly clock without cryptochrome

AU - Kistenpfennig, Christa

AU - Hirsh, Jay

AU - Yoshii, Taishi

AU - Helfrich-Förster, Charlotte

PY - 2012/4

Y1 - 2012/4

N2 - The blue light photopigment cryptochrome (CRY) is thought to be the main circadian photoreceptor of Drosophila melanogaster. Nevertheless, entrainment to light-dark cycles is possible without functional CRY. Here, we monitored phase response curves of cry01 mutants and control flies to 1-hour 1000-lux light pulses. We found that cry01 mutants phase-shift their activity rhythm in the subjective early morning and late evening, although with reduced magnitude. This phase-shifting capability is sufficient for the slowed entrainment of the mutants, indicating that the eyes contribute to the clock's light sensitivity around dawn and dusk. With longer light pulses (3 hours and 6 hours), wild-type flies show greatly enhanced magnitude of phase shift, but CRY-less flies seem impaired in the ability to integrate duration of the light pulse in a wild-type manner: Only 6-hour light pulses at circadian time 21 significantly increased the magnitude of phase advances in cry01 mutants. At circadian time 15, the mutants exhibited phase advances instead of the expected delays. These complex results are discussed.

AB - The blue light photopigment cryptochrome (CRY) is thought to be the main circadian photoreceptor of Drosophila melanogaster. Nevertheless, entrainment to light-dark cycles is possible without functional CRY. Here, we monitored phase response curves of cry01 mutants and control flies to 1-hour 1000-lux light pulses. We found that cry01 mutants phase-shift their activity rhythm in the subjective early morning and late evening, although with reduced magnitude. This phase-shifting capability is sufficient for the slowed entrainment of the mutants, indicating that the eyes contribute to the clock's light sensitivity around dawn and dusk. With longer light pulses (3 hours and 6 hours), wild-type flies show greatly enhanced magnitude of phase shift, but CRY-less flies seem impaired in the ability to integrate duration of the light pulse in a wild-type manner: Only 6-hour light pulses at circadian time 21 significantly increased the magnitude of phase advances in cry01 mutants. At circadian time 15, the mutants exhibited phase advances instead of the expected delays. These complex results are discussed.

KW - cryptochrome

KW - Drosophila melanogaster

KW - light pulses

KW - locomotor activity

UR - http://www.scopus.com/inward/record.url?scp=84859498645&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859498645&partnerID=8YFLogxK

U2 - 10.1177/0748730411434390

DO - 10.1177/0748730411434390

M3 - Article

VL - 27

SP - 117

EP - 125

JO - Journal of Biological Rhythms

JF - Journal of Biological Rhythms

SN - 0748-7304

IS - 2

ER -