pH profile of cytochrome c-catalyzed tyrosine nitration

Yasuhiro Kambayashi, Yoshiaki Hitomi, Norio Kodama, Masayuki Kubo, Junna Okuda, Kei Takemoto, Masafumi Shibamori, Tomoko Takigawa, Keiki Ogino

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In the present study, we investigated how cytochrome c catalyzed the nitration of tyrosine at various pHs. The cytochrome c-catalyzed nitration of tyrosine occurred in proportion to the concentration of hydrogen peroxide, nitrite or cytochrome c. The cytochrome c-catalyzed nitration of tyrosine was inhibited by catalase, sodium azide, cystein, and uric acid. These results show that the cytochrome c-catalyzed nitrotyrosine formation was due to peroxidase activity. The rate constant between cytochrome c and hydrogen peroxide within the pH range of 3-8 was the largest at pH 6 (37°C). The amount of nitrotyrosine formed was the greatest at pH 5. At pH 3, only cytochrome c-independent nitration of tyrosine occurred in the presence of nitrite. At this pH, the UV as well as visible spectrum of cytochrome c was changed by nitrite, even in the presence of hydrogen peroxide, probably via the formation of a heme iron-nitric oxide complex. Due to this change, the peroxidase activity of cytochrome c was lost.

Original languageEnglish
Pages (from-to)577-584
Number of pages8
JournalActa Biochimica Polonica
Volume53
Issue number3
Publication statusPublished - 2006

Fingerprint

Nitration
Cytochromes c
Tyrosine
Nitrites
Hydrogen Peroxide
Cytochrome-c Peroxidase
Peroxidase
Sodium Azide
Uric Acid
Heme
Catalase
Nitric Oxide
Iron oxides
Rate constants

Keywords

  • Cytochrome c
  • Nitrite
  • Nitrotyrosine
  • pH
  • Pseudo-peroxidase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Kambayashi, Y., Hitomi, Y., Kodama, N., Kubo, M., Okuda, J., Takemoto, K., ... Ogino, K. (2006). pH profile of cytochrome c-catalyzed tyrosine nitration. Acta Biochimica Polonica, 53(3), 577-584.

pH profile of cytochrome c-catalyzed tyrosine nitration. / Kambayashi, Yasuhiro; Hitomi, Yoshiaki; Kodama, Norio; Kubo, Masayuki; Okuda, Junna; Takemoto, Kei; Shibamori, Masafumi; Takigawa, Tomoko; Ogino, Keiki.

In: Acta Biochimica Polonica, Vol. 53, No. 3, 2006, p. 577-584.

Research output: Contribution to journalArticle

Kambayashi, Y, Hitomi, Y, Kodama, N, Kubo, M, Okuda, J, Takemoto, K, Shibamori, M, Takigawa, T & Ogino, K 2006, 'pH profile of cytochrome c-catalyzed tyrosine nitration', Acta Biochimica Polonica, vol. 53, no. 3, pp. 577-584.
Kambayashi Y, Hitomi Y, Kodama N, Kubo M, Okuda J, Takemoto K et al. pH profile of cytochrome c-catalyzed tyrosine nitration. Acta Biochimica Polonica. 2006;53(3):577-584.
Kambayashi, Yasuhiro ; Hitomi, Yoshiaki ; Kodama, Norio ; Kubo, Masayuki ; Okuda, Junna ; Takemoto, Kei ; Shibamori, Masafumi ; Takigawa, Tomoko ; Ogino, Keiki. / pH profile of cytochrome c-catalyzed tyrosine nitration. In: Acta Biochimica Polonica. 2006 ; Vol. 53, No. 3. pp. 577-584.
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