pH-Induced Regulation of Excitation Energy Transfer in the Cyanobacterial Photosystem I Tetramer

Ryo Nagao, Makio Yokono, Yoshifumi Ueno, Tian Yi Jiang, Jian Ren Shen, Seiji Akimoto

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Excitation energy-transfer processes in pigment-protein complexes in photosynthetic organisms are often changed under different pH conditions. However, it is unclear how the pH changes affect excitation energy relaxations in photosystem I (PSI) cores. In this study, we examined the pH sensitivity of energy dynamics in the PSI tetramer, dimer, and monomer isolated from a cyanobacterium, Anabaena sp. PCC 7120, by means of time-resolved fluorescence spectroscopy. Each PSI was adapted to pH 5.0, 6.5, and 8.0. Fluorescence decay-associated (FDA) spectra of the pH 8.0 PSI dimer and monomer showed positive and negative peaks within 5 ps, whereas the FDA spectra of the PSI tetramer did not show such a 5 ps fluorescence component. Mean lifetimes of the fluorescence at pH 6.5 are shorter in the PSI tetramer than in the PSI dimer and monomer, indicating an accelerated energy quenching in the tetramer. The effects of the acidic and basic pHs on the energy-transfer processes differ significantly among the three types of PSIs, suggesting different pH-sensing sites around pigment molecules in the three PSIs. Based on these results, together with our recent structural finding of the PSI tetramer, we discuss functional implications for the pH-sensing regulation of the excitation energy transfer in the PSI tetramer.

Original languageEnglish
Pages (from-to)1949-1954
Number of pages6
JournalJournal of Physical Chemistry B
Volume124
Issue number10
DOIs
Publication statusPublished - Mar 12 2020

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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