pEYFP-Nuc vector is a useful tool for three-dimensional and time-lapse observation of nuclear morphology of Jurkat cells during apoptosis.

Akira Hasegawa, Masahiro Kuroda, Yadi Wang, Shiro Akaki, Jun-Ichi Asaumi, Koichi Shibuya, Shoji Kawasaki, Yoshihiro Okumura, Susumu Kanazawa, Yoshio Hiraki

Research output: Contribution to journalArticle

Abstract

Using a pEYFP-Nuc vector, which contains nucleic acid sequences of a nuclear localization signal, we established a Jurkat-YN cell line that expressed enhanced yellow fluorescent protein (EYFP) in the nucleus. We observed three-dimensional and time-lapse changes in nuclear morphology of Jurkat-YN cells during Fas-induced apoptosis using a confocal laser scanning microscope. The nuclear forms visualized by EYFP were almost equal in quality to those visualized by SYTO59, a nucleic acid stain for living cells. Three-dimensional deformities in the nuclear form were observed during apoptosis before chromatin condensation became apparent, indicating these deformities are characteristic morphological changes of the early stage of apoptosis. In conclusion, the pEYFP-Nuc vector is a useful tool in the time-lapse observation of nuclear morphology of living cells during apoptosis.

Original languageEnglish
Pages (from-to)235-242
Number of pages8
JournalInternational Journal of Molecular Medicine
Volume13
Issue number2
Publication statusPublished - Feb 2004

Fingerprint

Jurkat Cells
Observation
Apoptosis
Nucleic Acids
Nuclear Localization Signals
Chromatin
Proteins
Lasers
Coloring Agents
Cell Line

ASJC Scopus subject areas

  • Genetics

Cite this

pEYFP-Nuc vector is a useful tool for three-dimensional and time-lapse observation of nuclear morphology of Jurkat cells during apoptosis. / Hasegawa, Akira; Kuroda, Masahiro; Wang, Yadi; Akaki, Shiro; Asaumi, Jun-Ichi; Shibuya, Koichi; Kawasaki, Shoji; Okumura, Yoshihiro; Kanazawa, Susumu; Hiraki, Yoshio.

In: International Journal of Molecular Medicine, Vol. 13, No. 2, 02.2004, p. 235-242.

Research output: Contribution to journalArticle

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abstract = "Using a pEYFP-Nuc vector, which contains nucleic acid sequences of a nuclear localization signal, we established a Jurkat-YN cell line that expressed enhanced yellow fluorescent protein (EYFP) in the nucleus. We observed three-dimensional and time-lapse changes in nuclear morphology of Jurkat-YN cells during Fas-induced apoptosis using a confocal laser scanning microscope. The nuclear forms visualized by EYFP were almost equal in quality to those visualized by SYTO59, a nucleic acid stain for living cells. Three-dimensional deformities in the nuclear form were observed during apoptosis before chromatin condensation became apparent, indicating these deformities are characteristic morphological changes of the early stage of apoptosis. In conclusion, the pEYFP-Nuc vector is a useful tool in the time-lapse observation of nuclear morphology of living cells during apoptosis.",
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AU - Hasegawa, Akira

AU - Kuroda, Masahiro

AU - Wang, Yadi

AU - Akaki, Shiro

AU - Asaumi, Jun-Ichi

AU - Shibuya, Koichi

AU - Kawasaki, Shoji

AU - Okumura, Yoshihiro

AU - Kanazawa, Susumu

AU - Hiraki, Yoshio

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