PET imaging of hypoxia-inducible factor-1-active tumor cells with pretargeted oxygen-dependent degradable streptavidin and a novel 18F-labeled biotin derivative Takashi Kudo,1 Masashi Ueda,1,2 Hiroaki Konishi,1 Hidekazu Kawashima,1,3

Takashi Kudo, Masashi Ueda, Hiroaki Konishi, Hidekazu Kawashima, Yuji Kuge, Takahiro Mukai, Azusa Miyano, Shotaro Tanaka, Shinae Kizaka-Kondoh, Masahiro Hiraoka, Hideo Saji

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Purpose: We aimed to evaluate the feasibility of using streptavidin-biotin- based pretargeting for positron emission tomography (PET) imaging of hypoxia-inducible factor (HIF)-1-active tumors. Procedures: We used POS, a genetically engineered form of streptavidin that selectively stabilizes in HIF-1-active cells, and (4- 18F-fluorobenzoyl)norbiotinamide ( 18F-FBB), a radiolabeled biotin derivative, for performing a biodistribution study and for PET imaging. The tumoral 18F-FBB accumulation was compared to the HIF-1-dependent luciferase bioluminescence and HIF-1a immunohistochemical signal. Results: 18F-FBB accumulation was observed in POS-pretargeted tumors in mice (2.85±0.55% injected dose per gram at 3 h), and clear PET images were obtained at the same time point. The tumoral 18F-FBB accumulation positively correlated with luciferase bioluminescence (R=0.72, P18F-FBB accumulation corresponded to HIF-1a-positive areas. Conclusion: Pretargeting with POS and 18F-FBB is an effective approach for PET imaging of HIF-1-active areas in tumors.

Original languageEnglish
Pages (from-to)1003-1010
Number of pages8
JournalMolecular Imaging and Biology
Volume13
Issue number5
DOIs
Publication statusPublished - Oct 2011
Externally publishedYes

Fingerprint

Hypoxia-Inducible Factor 1
Streptavidin
Biotin
Positron-Emission Tomography
Oxygen
Luciferases
Neoplasms
Hypoxia

Keywords

  • F-labeled biotin derivative
  • Hypoxia-inducible factor-1 (HIF-1)
  • Oxygen-dependent degradation domain (ODD)
  • Pretargeting
  • Tumor hypoxia

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Radiology Nuclear Medicine and imaging

Cite this

PET imaging of hypoxia-inducible factor-1-active tumor cells with pretargeted oxygen-dependent degradable streptavidin and a novel 18F-labeled biotin derivative Takashi Kudo,1 Masashi Ueda,1,2 Hiroaki Konishi,1 Hidekazu Kawashima,1,3. / Kudo, Takashi; Ueda, Masashi; Konishi, Hiroaki; Kawashima, Hidekazu; Kuge, Yuji; Mukai, Takahiro; Miyano, Azusa; Tanaka, Shotaro; Kizaka-Kondoh, Shinae; Hiraoka, Masahiro; Saji, Hideo.

In: Molecular Imaging and Biology, Vol. 13, No. 5, 10.2011, p. 1003-1010.

Research output: Contribution to journalArticle

Kudo, Takashi ; Ueda, Masashi ; Konishi, Hiroaki ; Kawashima, Hidekazu ; Kuge, Yuji ; Mukai, Takahiro ; Miyano, Azusa ; Tanaka, Shotaro ; Kizaka-Kondoh, Shinae ; Hiraoka, Masahiro ; Saji, Hideo. / PET imaging of hypoxia-inducible factor-1-active tumor cells with pretargeted oxygen-dependent degradable streptavidin and a novel 18F-labeled biotin derivative Takashi Kudo,1 Masashi Ueda,1,2 Hiroaki Konishi,1 Hidekazu Kawashima,1,3. In: Molecular Imaging and Biology. 2011 ; Vol. 13, No. 5. pp. 1003-1010.
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AU - Ueda, Masashi

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AU - Miyano, Azusa

AU - Tanaka, Shotaro

AU - Kizaka-Kondoh, Shinae

AU - Hiraoka, Masahiro

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