Peroxisomes exist in growth cones and move anterogradely and retrogradely in neurites of PC12D cells

Tetsuya Ishikawa, Chikage Kawai, Mamoru Sano, Yohsuke Minatogawa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Localization and movement of peroxisomes have been investigated in neurites of a subline of PC12 pheochromocytoma cells (PC12D cells). The cells were transfected with a construct encoding the green fluorescent protein and bearing the C-terminal peroxisomal targeting signal 1 SKL motif (-Ser-Lys-Leu-COOH). Peroxisomes were detected as green punctate fluorescent signals. Many peroxisomes were observed in neurites of PC12D cells, especially in neural terminal-like structures, growth cones, varicosities, and branch points. Growth cones containing many peroxisomes were active, since they extended several long filopodias. Existence of peroxisomes in growth cones and neuronal terminal-like structures suggests that peroxisomes might have some role in neuronal extension and nerve terminal functioning. Peroxisomal motility was analyzed by time-lapse imaging using a fluorescence microscope at 25°C. Peroxisomes were transported bidirectionally in neurites, i.e., through anterograde and retrograde transport. This result suggests that peroxisomes move to growth cones and neural terminals from the PC12D cell body, play some role in these parts, and go back to cell body.

Original languageEnglish
Pages (from-to)260-269
Number of pages10
JournalExperimental Cell Research
Volume266
Issue number2
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Growth Cones
Peroxisomes
Neurites
seryl-lysyl-leucine
Time-Lapse Imaging
PC12 Cells
Pheochromocytoma
Protein C
Green Fluorescent Proteins
Fluorescence

Keywords

  • Anterograde transport
  • GFP
  • Growth cone
  • Motility
  • Neurite
  • Peroxisome
  • Retrograde transport

ASJC Scopus subject areas

  • Cell Biology

Cite this

Peroxisomes exist in growth cones and move anterogradely and retrogradely in neurites of PC12D cells. / Ishikawa, Tetsuya; Kawai, Chikage; Sano, Mamoru; Minatogawa, Yohsuke.

In: Experimental Cell Research, Vol. 266, No. 2, 2001, p. 260-269.

Research output: Contribution to journalArticle

Ishikawa, Tetsuya ; Kawai, Chikage ; Sano, Mamoru ; Minatogawa, Yohsuke. / Peroxisomes exist in growth cones and move anterogradely and retrogradely in neurites of PC12D cells. In: Experimental Cell Research. 2001 ; Vol. 266, No. 2. pp. 260-269.
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