Peroxiredoxin family proteins are key initiators of post-ischemic inflammation in the brain

Takashi Shichita, Eiichi Hasegawa, Akihiro Kimura, Rimpei Morita, Ryota Sakaguchi, Ichiro Takada, Takashi Sekiya, Hiroaki Ooboshi, Takanari Kitazono, Toru Yanagawa, Tetsuro Ishii, Hideo Takahashi, Shuji Mori, Masahiro Nishibori, Kazumichi Kuroda, Shizuo Akira, Kensuke Miyake, Akihiko Yoshimura

Research output: Contribution to journalArticle

217 Citations (Scopus)

Abstract

Post-ischemic inflammation is an essential step in the progression of brain ischemia-reperfusion injury. However, the mechanism that activates infiltrating macrophages in the ischemic brain remains to be clarified. Here we demonstrate that peroxiredoxin (Prx) family proteins released extracellularly from necrotic brain cells induce expression of inflammatory cytokines including interleukin-23 in macrophages through activation of Toll-like receptor 2 (TLR2) and TLR4, thereby promoting neural cell death, even though intracellular Prxs have been shown to be neuroprotective. The extracellular release of Prxs in the ischemic core occurred 12 h after stroke onset, and neutralization of extracellular Prxs with antibodies suppressed inflammatory cytokine expression and infarct volume growth. In contrast, high mobility group box 1 (HMGB1), a well-known damage-associated molecular pattern molecule, was released before Prx and had a limited role in post-ischemic macrophage activation. We thus propose that extracellular Prxs are previously unknown danger signals in the ischemic brain and that its blocking agents are potent neuroprotective tools.

Original languageEnglish
Pages (from-to)911-917
Number of pages7
JournalNature Medicine
Volume18
Issue number6
DOIs
Publication statusPublished - Jun 2012

Fingerprint

Peroxiredoxins
Encephalitis
Macrophages
Brain
Macrophage Activation
Cytokines
Interleukin-23
Toll-Like Receptor 2
Proteins
Chemical activation
Neuroprotective Agents
Reperfusion Injury
Brain Ischemia
Cell Death
Cell death
Stroke
Inflammation
Antibodies
Growth

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Shichita, T., Hasegawa, E., Kimura, A., Morita, R., Sakaguchi, R., Takada, I., ... Yoshimura, A. (2012). Peroxiredoxin family proteins are key initiators of post-ischemic inflammation in the brain. Nature Medicine, 18(6), 911-917. https://doi.org/10.1038/nm.2749

Peroxiredoxin family proteins are key initiators of post-ischemic inflammation in the brain. / Shichita, Takashi; Hasegawa, Eiichi; Kimura, Akihiro; Morita, Rimpei; Sakaguchi, Ryota; Takada, Ichiro; Sekiya, Takashi; Ooboshi, Hiroaki; Kitazono, Takanari; Yanagawa, Toru; Ishii, Tetsuro; Takahashi, Hideo; Mori, Shuji; Nishibori, Masahiro; Kuroda, Kazumichi; Akira, Shizuo; Miyake, Kensuke; Yoshimura, Akihiko.

In: Nature Medicine, Vol. 18, No. 6, 06.2012, p. 911-917.

Research output: Contribution to journalArticle

Shichita, T, Hasegawa, E, Kimura, A, Morita, R, Sakaguchi, R, Takada, I, Sekiya, T, Ooboshi, H, Kitazono, T, Yanagawa, T, Ishii, T, Takahashi, H, Mori, S, Nishibori, M, Kuroda, K, Akira, S, Miyake, K & Yoshimura, A 2012, 'Peroxiredoxin family proteins are key initiators of post-ischemic inflammation in the brain', Nature Medicine, vol. 18, no. 6, pp. 911-917. https://doi.org/10.1038/nm.2749
Shichita T, Hasegawa E, Kimura A, Morita R, Sakaguchi R, Takada I et al. Peroxiredoxin family proteins are key initiators of post-ischemic inflammation in the brain. Nature Medicine. 2012 Jun;18(6):911-917. https://doi.org/10.1038/nm.2749
Shichita, Takashi ; Hasegawa, Eiichi ; Kimura, Akihiro ; Morita, Rimpei ; Sakaguchi, Ryota ; Takada, Ichiro ; Sekiya, Takashi ; Ooboshi, Hiroaki ; Kitazono, Takanari ; Yanagawa, Toru ; Ishii, Tetsuro ; Takahashi, Hideo ; Mori, Shuji ; Nishibori, Masahiro ; Kuroda, Kazumichi ; Akira, Shizuo ; Miyake, Kensuke ; Yoshimura, Akihiko. / Peroxiredoxin family proteins are key initiators of post-ischemic inflammation in the brain. In: Nature Medicine. 2012 ; Vol. 18, No. 6. pp. 911-917.
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