Peroxide production and apoptosis in cultured cells carrying mtDNA mutation causing encephalomyopathy

Jin Zhang, Makoto Yoneda, Keiji Naruse, Harm Jan W. Borgeld, Jian Sheng Gong, Shu Ichi Obata, Masashi Tanaka, Kunio Yagi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

When cybrids with a point mutation, which locates in the tRNA(Leu(UUR)) gene of mtDNA and causes a mitochondrial encephalomyopathy (MELAS syndrome), were exposed to a high concentration of oxygen (95%), the peroxide production markedly increased by 6 h of oxygen exposure, whereas the peroxide production was similar among the cybrids under a normal concentration of oxygen. The peroxide production by oxygen exposure was enhanced particularly in cybrids with high proportions of the mutant mtDNA and low respiratory capacities. The appearance of apoptotic cells by oxygen exposure was high in cybrids with the impaired respiratory function due to the mutation. An antioxidant NAC successfully suppressed both the peroxide production and apoptosis. These results imply that the peroxide production plays an important role in inducing apoptosis in cells carrying the mtDNA mutation causing encephalomyopathy.

Original languageEnglish
Pages (from-to)71-79
Number of pages9
JournalBiochemistry and Molecular Biology International
Volume46
Issue number1
DOIs
Publication statusPublished - 1998
Externally publishedYes

Keywords

  • Apoptosis
  • MELAS
  • Peoxide production
  • mtDNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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