Peroxidase-like catalytic activity of Mn- and Fe-tetrakis(4-carboxyphenyl) porphines bound to aminopropyl-glass bead in oxidative reaction of heterocyclic amines

Youji Kitamura, Masaki Mifune, Daiki Hino, Saori Yokotani, Madoka Saito, Ikuko Tsukamoto, Akimasa Iwado, Yutaka Saito

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Fe- or Mn-tetrakis(4-carboxyphenyl)porphine (Fe- and Mn-TCPP) bound to aminopropyl-glass bead (Fe- and Mn-TCPPgs) was examined for the peroxidase (POD)-like function in order to develop a solid catalyst which can exhibit POD-like activity without adsorbing heterocyclic amines (HCAs). Mn-TCPP in aqueous solution had only a slight POD-like catalytic activity on HCAs (IQ and MeIQ). As for Fe-TCPP, it was impossible to examine the POD-like activity since it reacted with hydrogen peroxide in a liquid reaction system. However, both Fe- and Mn-TCPP when immobilized on aminopropyl-glass bead via peptide bond (Fe- and Mn-TCPPgs), catalyzed the oxidative reaction of mutagenic HCAs with hydrogen peroxide. The catalytic activity of Fe- and Mn-TCPP gs was investigated in more detail using as a substrate IQ and MeIQ which were oxidized more rapidly among the tested HCAs. Consequently, the optimal conditions for the oxidative reaction catalyzed by Fe- and Mn-TCPP gs were determined. In addition, ESI-mass and absorption spectra of oxidation products of IQ and MeIQ showed that they are dimers. Thus, it was demonstrated that a solid catalyst with POD-like activity can be obtained by immobilizing Fe- and Mn-TCPPs on aminopropyl-glass beads.

Original languageEnglish
Pages (from-to)43-47
Number of pages5
JournalTalanta
Volume69
Issue number1
DOIs
Publication statusPublished - Mar 15 2006

Fingerprint

2-amino-3,4-dimethylimidazo(4,5-f)quinoline
Peroxidase
Amines
Glass
Catalyst activity
Hydrogen Peroxide
Catalysts
Dimers
manganese(III)-tetra(4-carboxyphenyl)porphine
iron 5,10,15,20-tetrakis-4-carboxyphenylporphyrin
ferryl iron
Absorption spectra
Oxidation
Peptides
Liquids
Substrates

Keywords

  • Glass-bead
  • Heterocyclic amine
  • Immobilization
  • Metal-porphyrin
  • Peroxidase-like activity

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Peroxidase-like catalytic activity of Mn- and Fe-tetrakis(4-carboxyphenyl) porphines bound to aminopropyl-glass bead in oxidative reaction of heterocyclic amines. / Kitamura, Youji; Mifune, Masaki; Hino, Daiki; Yokotani, Saori; Saito, Madoka; Tsukamoto, Ikuko; Iwado, Akimasa; Saito, Yutaka.

In: Talanta, Vol. 69, No. 1, 15.03.2006, p. 43-47.

Research output: Contribution to journalArticle

Kitamura, Youji ; Mifune, Masaki ; Hino, Daiki ; Yokotani, Saori ; Saito, Madoka ; Tsukamoto, Ikuko ; Iwado, Akimasa ; Saito, Yutaka. / Peroxidase-like catalytic activity of Mn- and Fe-tetrakis(4-carboxyphenyl) porphines bound to aminopropyl-glass bead in oxidative reaction of heterocyclic amines. In: Talanta. 2006 ; Vol. 69, No. 1. pp. 43-47.
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AU - Yokotani, Saori

AU - Saito, Madoka

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AU - Iwado, Akimasa

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