Abstract
Fe- or Mn-tetrakis(4-carboxyphenyl)porphine (Fe- and Mn-TCPP) bound to aminopropyl-glass bead (Fe- and Mn-TCPPgs) was examined for the peroxidase (POD)-like function in order to develop a solid catalyst which can exhibit POD-like activity without adsorbing heterocyclic amines (HCAs). Mn-TCPP in aqueous solution had only a slight POD-like catalytic activity on HCAs (IQ and MeIQ). As for Fe-TCPP, it was impossible to examine the POD-like activity since it reacted with hydrogen peroxide in a liquid reaction system. However, both Fe- and Mn-TCPP when immobilized on aminopropyl-glass bead via peptide bond (Fe- and Mn-TCPPgs), catalyzed the oxidative reaction of mutagenic HCAs with hydrogen peroxide. The catalytic activity of Fe- and Mn-TCPP gs was investigated in more detail using as a substrate IQ and MeIQ which were oxidized more rapidly among the tested HCAs. Consequently, the optimal conditions for the oxidative reaction catalyzed by Fe- and Mn-TCPP gs were determined. In addition, ESI-mass and absorption spectra of oxidation products of IQ and MeIQ showed that they are dimers. Thus, it was demonstrated that a solid catalyst with POD-like activity can be obtained by immobilizing Fe- and Mn-TCPPs on aminopropyl-glass beads.
| Original language | English |
|---|---|
| Pages (from-to) | 43-47 |
| Number of pages | 5 |
| Journal | Talanta |
| Volume | 69 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Mar 15 2006 |
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Keywords
- Glass-bead
- Heterocyclic amine
- Immobilization
- Metal-porphyrin
- Peroxidase-like activity
ASJC Scopus subject areas
- Analytical Chemistry
- Spectroscopy
Cite this
Peroxidase-like catalytic activity of Mn- and Fe-tetrakis(4-carboxyphenyl) porphines bound to aminopropyl-glass bead in oxidative reaction of heterocyclic amines. / Kitamura, Youji; Mifune, Masaki; Hino, Daiki; Yokotani, Saori; Saito, Madoka; Tsukamoto, Ikuko; Iwado, Akimasa; Saito, Yutaka.
In: Talanta, Vol. 69, No. 1, 15.03.2006, p. 43-47.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Peroxidase-like catalytic activity of Mn- and Fe-tetrakis(4-carboxyphenyl) porphines bound to aminopropyl-glass bead in oxidative reaction of heterocyclic amines
AU - Kitamura, Youji
AU - Mifune, Masaki
AU - Hino, Daiki
AU - Yokotani, Saori
AU - Saito, Madoka
AU - Tsukamoto, Ikuko
AU - Iwado, Akimasa
AU - Saito, Yutaka
PY - 2006/3/15
Y1 - 2006/3/15
N2 - Fe- or Mn-tetrakis(4-carboxyphenyl)porphine (Fe- and Mn-TCPP) bound to aminopropyl-glass bead (Fe- and Mn-TCPPgs) was examined for the peroxidase (POD)-like function in order to develop a solid catalyst which can exhibit POD-like activity without adsorbing heterocyclic amines (HCAs). Mn-TCPP in aqueous solution had only a slight POD-like catalytic activity on HCAs (IQ and MeIQ). As for Fe-TCPP, it was impossible to examine the POD-like activity since it reacted with hydrogen peroxide in a liquid reaction system. However, both Fe- and Mn-TCPP when immobilized on aminopropyl-glass bead via peptide bond (Fe- and Mn-TCPPgs), catalyzed the oxidative reaction of mutagenic HCAs with hydrogen peroxide. The catalytic activity of Fe- and Mn-TCPP gs was investigated in more detail using as a substrate IQ and MeIQ which were oxidized more rapidly among the tested HCAs. Consequently, the optimal conditions for the oxidative reaction catalyzed by Fe- and Mn-TCPP gs were determined. In addition, ESI-mass and absorption spectra of oxidation products of IQ and MeIQ showed that they are dimers. Thus, it was demonstrated that a solid catalyst with POD-like activity can be obtained by immobilizing Fe- and Mn-TCPPs on aminopropyl-glass beads.
AB - Fe- or Mn-tetrakis(4-carboxyphenyl)porphine (Fe- and Mn-TCPP) bound to aminopropyl-glass bead (Fe- and Mn-TCPPgs) was examined for the peroxidase (POD)-like function in order to develop a solid catalyst which can exhibit POD-like activity without adsorbing heterocyclic amines (HCAs). Mn-TCPP in aqueous solution had only a slight POD-like catalytic activity on HCAs (IQ and MeIQ). As for Fe-TCPP, it was impossible to examine the POD-like activity since it reacted with hydrogen peroxide in a liquid reaction system. However, both Fe- and Mn-TCPP when immobilized on aminopropyl-glass bead via peptide bond (Fe- and Mn-TCPPgs), catalyzed the oxidative reaction of mutagenic HCAs with hydrogen peroxide. The catalytic activity of Fe- and Mn-TCPP gs was investigated in more detail using as a substrate IQ and MeIQ which were oxidized more rapidly among the tested HCAs. Consequently, the optimal conditions for the oxidative reaction catalyzed by Fe- and Mn-TCPP gs were determined. In addition, ESI-mass and absorption spectra of oxidation products of IQ and MeIQ showed that they are dimers. Thus, it was demonstrated that a solid catalyst with POD-like activity can be obtained by immobilizing Fe- and Mn-TCPPs on aminopropyl-glass beads.
KW - Glass-bead
KW - Heterocyclic amine
KW - Immobilization
KW - Metal-porphyrin
KW - Peroxidase-like activity
UR - http://www.scopus.com/inward/record.url?scp=31544466230&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=31544466230&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2005.08.045
DO - 10.1016/j.talanta.2005.08.045
M3 - Article
VL - 69
SP - 43
EP - 47
JO - Talanta
JF - Talanta
SN - 0039-9140
IS - 1
ER -