Permeability of a sol-gel synthesized aminopropyl-silicate-titanate hybrid membrane for the microcapsule-shaped bioartificial pancreas

S. Sakai, T. Ono, H. Ijima, K. Kawakami

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The aminopropyl-silicate-titanate hybrid membrane was successfully synthesized onto a Ca-alginate gel bead by the sol-gel method, from two kinds of organometallic compound precursors, tetraethyl orthotitanate (TEOTi) [Ti(OC2H5)4] and 3-aminopropyltrimethyl orthosilicate (APTrMOS) [(CH3O)3Si(CH2)3NH2]. APTrMOS acted as a binding agent between inorganic titanate and alginate polymer via electrostatic interaction between amino groups of APTrMOS and carboxyl groups of alginate. From the measurements of the diffusion of ovalbumin and γ-globulin through the aminopropyl-silicate-titanate hybrid membrane, it was found that the molecular permeability of the membrane could be controlled by changing the amount of each precursor. The molecular weight cutoff point required for immunoisolation, less than 150kDa, was achieved under the condition that the amount of APTrMOS was 3.40 mmol/ (10 ml of Ca-alginate) and TEOTi was more than 1.42 mmol. The islets encapsulated in the membrane showed almost the same insulin secretion viability as the free islets.

Original languageEnglish
Pages (from-to)54-59
Number of pages6
JournalJournal of Artificial Organs
Volume5
Issue number1
DOIs
Publication statusPublished - Jan 1 2002

Keywords

  • Artificial pancreas
  • Immunoisolation
  • Microcapsule
  • Silicate-titanate hybrid
  • Sol-gel method

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering
  • Cardiology and Cardiovascular Medicine

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