Abstract
Nuclear HMGB1 that contains 3 cysteine residues is acetylated and secreted to the extracellular space, promoting inflammation via multiple molecules such as RAGE and TLR4. We thus evaluated and characterized the redox state-dependent effects of peripheral HMGB1 on nociception. Intraplantar (i.pl.) administration of bovine thymus-derived HMGB1 (bt-HMGB1), all-thiol HMGB1 (at-HMGB1) or disulfide HMGB1 (ds-HMGB1) caused long-lasting mechanical hyperalgesia in mice. The hyperalgesia following i.pl. bt-HMGB1 or at-HMGB1 was attenuated by RAGE inhibitors, while the ds-HMGB1-induced hyperalgesia was abolished by a TLR4 antagonist. Thus, nociceptive processing by peripheral HMGB1 is considered dependent on its redox states.
| Original language | English |
|---|---|
| Journal | Journal of Pharmacological Sciences |
| DOIs | |
| Publication status | Accepted/In press - Nov 23 2015 |
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Keywords
- High mobility group box 1
- Pain
- Redox state
ASJC Scopus subject areas
- Pharmacology
- Molecular Medicine
Cite this
Peripheral HMGB1-induced hyperalgesia in mice : Redox state-dependent distinct roles of RAGE and TLR4. / Yamasoba, Daichi; Tsubota, Maho; Domoto, Risa; Sekiguchi, Fumiko; Nishikawa, Hiroyuki; Liu, Keyue; Nishibori, Masahiro; Ishikura, Hiroyasu; Yamamoto, Tetsushi; Taga, Atsushi; Kawabata, Atsufumi.
In: Journal of Pharmacological Sciences, 23.11.2015.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Peripheral HMGB1-induced hyperalgesia in mice
T2 - Redox state-dependent distinct roles of RAGE and TLR4
AU - Yamasoba, Daichi
AU - Tsubota, Maho
AU - Domoto, Risa
AU - Sekiguchi, Fumiko
AU - Nishikawa, Hiroyuki
AU - Liu, Keyue
AU - Nishibori, Masahiro
AU - Ishikura, Hiroyasu
AU - Yamamoto, Tetsushi
AU - Taga, Atsushi
AU - Kawabata, Atsufumi
PY - 2015/11/23
Y1 - 2015/11/23
N2 - Nuclear HMGB1 that contains 3 cysteine residues is acetylated and secreted to the extracellular space, promoting inflammation via multiple molecules such as RAGE and TLR4. We thus evaluated and characterized the redox state-dependent effects of peripheral HMGB1 on nociception. Intraplantar (i.pl.) administration of bovine thymus-derived HMGB1 (bt-HMGB1), all-thiol HMGB1 (at-HMGB1) or disulfide HMGB1 (ds-HMGB1) caused long-lasting mechanical hyperalgesia in mice. The hyperalgesia following i.pl. bt-HMGB1 or at-HMGB1 was attenuated by RAGE inhibitors, while the ds-HMGB1-induced hyperalgesia was abolished by a TLR4 antagonist. Thus, nociceptive processing by peripheral HMGB1 is considered dependent on its redox states.
AB - Nuclear HMGB1 that contains 3 cysteine residues is acetylated and secreted to the extracellular space, promoting inflammation via multiple molecules such as RAGE and TLR4. We thus evaluated and characterized the redox state-dependent effects of peripheral HMGB1 on nociception. Intraplantar (i.pl.) administration of bovine thymus-derived HMGB1 (bt-HMGB1), all-thiol HMGB1 (at-HMGB1) or disulfide HMGB1 (ds-HMGB1) caused long-lasting mechanical hyperalgesia in mice. The hyperalgesia following i.pl. bt-HMGB1 or at-HMGB1 was attenuated by RAGE inhibitors, while the ds-HMGB1-induced hyperalgesia was abolished by a TLR4 antagonist. Thus, nociceptive processing by peripheral HMGB1 is considered dependent on its redox states.
KW - High mobility group box 1
KW - Pain
KW - Redox state
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UR - http://www.scopus.com/inward/citedby.url?scp=84957646711&partnerID=8YFLogxK
U2 - 10.1016/j.jphs.2016.01.005
DO - 10.1016/j.jphs.2016.01.005
M3 - Article
C2 - 26883456
AN - SCOPUS:84957646711
JO - Journal of Pharmacological Sciences
JF - Journal of Pharmacological Sciences
SN - 1347-8648
ER -