Peripheral circadian clock for the cuticle deposition rhythm in Drosophila melanogaster

Chihiro Ito, Shin G. Goto, Sakiko Shiga, Kenji Tomioka, Hideharu Numata

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Insect endocuticle thickens after adult emergence by daily alternating deposition of two chitin layers with different orientation. Although the cuticle deposition rhythm is known to be controlled by a circadian clock in many insects, the site of the driving clock, the photoreceptor for entrainment, and the oscillatory mechanism remain elusive. Here, we show that the cuticle deposition rhythm is regulated by a peripheral oscillator in the epidermis in Drosophila melanogaster. Free-running and entrainment experiments in vitro reveal that the oscillator for the cuticle deposition rhythm is independent of the central clock in the brain driving the locomotor rhythms. The cuticle deposition rhythm is absent in null and dominant-negative mutants of clock genes (i.e., period, timeless, cycle, and Clock), indicating that this oscillator is composed of the same clock genes as the central clock. Entrainment experiments with monochromatic light-dark cycles and cryb flies reveal that a blue light-absorbing photoreceptor, cryptochrome (CRY), acts as a photoreceptor pigment for the entrainment of the cuticle deposition rhythm. Unlike other peripheral rhythms in D. melanogaster, the cuticle deposition rhythm persisted in cryb and cryOUT mutant flies, indicating that CRY does not play a core role in the rhythm generation in the epidermal oscillator.

Original languageEnglish
Pages (from-to)8446-8451
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number24
DOIs
Publication statusPublished - Jun 17 2008

Fingerprint

Cryptochromes
Circadian Clocks
Drosophila melanogaster
Diptera
Insects
Chitin
Photoperiod
Epidermis
Running
Genes
Light
Brain

Keywords

  • Circadian rhythm
  • Clock genes
  • Cryptochrome
  • Entrainment
  • Epidermal cell

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Peripheral circadian clock for the cuticle deposition rhythm in Drosophila melanogaster. / Ito, Chihiro; Goto, Shin G.; Shiga, Sakiko; Tomioka, Kenji; Numata, Hideharu.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 24, 17.06.2008, p. 8446-8451.

Research output: Contribution to journalArticle

@article{b1117df299e94530b1655956881fdecc,
title = "Peripheral circadian clock for the cuticle deposition rhythm in Drosophila melanogaster",
abstract = "Insect endocuticle thickens after adult emergence by daily alternating deposition of two chitin layers with different orientation. Although the cuticle deposition rhythm is known to be controlled by a circadian clock in many insects, the site of the driving clock, the photoreceptor for entrainment, and the oscillatory mechanism remain elusive. Here, we show that the cuticle deposition rhythm is regulated by a peripheral oscillator in the epidermis in Drosophila melanogaster. Free-running and entrainment experiments in vitro reveal that the oscillator for the cuticle deposition rhythm is independent of the central clock in the brain driving the locomotor rhythms. The cuticle deposition rhythm is absent in null and dominant-negative mutants of clock genes (i.e., period, timeless, cycle, and Clock), indicating that this oscillator is composed of the same clock genes as the central clock. Entrainment experiments with monochromatic light-dark cycles and cryb flies reveal that a blue light-absorbing photoreceptor, cryptochrome (CRY), acts as a photoreceptor pigment for the entrainment of the cuticle deposition rhythm. Unlike other peripheral rhythms in D. melanogaster, the cuticle deposition rhythm persisted in cryb and cryOUT mutant flies, indicating that CRY does not play a core role in the rhythm generation in the epidermal oscillator.",
keywords = "Circadian rhythm, Clock genes, Cryptochrome, Entrainment, Epidermal cell",
author = "Chihiro Ito and Goto, {Shin G.} and Sakiko Shiga and Kenji Tomioka and Hideharu Numata",
year = "2008",
month = "6",
day = "17",
doi = "10.1073/pnas.0800145105",
language = "English",
volume = "105",
pages = "8446--8451",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "24",

}

TY - JOUR

T1 - Peripheral circadian clock for the cuticle deposition rhythm in Drosophila melanogaster

AU - Ito, Chihiro

AU - Goto, Shin G.

AU - Shiga, Sakiko

AU - Tomioka, Kenji

AU - Numata, Hideharu

PY - 2008/6/17

Y1 - 2008/6/17

N2 - Insect endocuticle thickens after adult emergence by daily alternating deposition of two chitin layers with different orientation. Although the cuticle deposition rhythm is known to be controlled by a circadian clock in many insects, the site of the driving clock, the photoreceptor for entrainment, and the oscillatory mechanism remain elusive. Here, we show that the cuticle deposition rhythm is regulated by a peripheral oscillator in the epidermis in Drosophila melanogaster. Free-running and entrainment experiments in vitro reveal that the oscillator for the cuticle deposition rhythm is independent of the central clock in the brain driving the locomotor rhythms. The cuticle deposition rhythm is absent in null and dominant-negative mutants of clock genes (i.e., period, timeless, cycle, and Clock), indicating that this oscillator is composed of the same clock genes as the central clock. Entrainment experiments with monochromatic light-dark cycles and cryb flies reveal that a blue light-absorbing photoreceptor, cryptochrome (CRY), acts as a photoreceptor pigment for the entrainment of the cuticle deposition rhythm. Unlike other peripheral rhythms in D. melanogaster, the cuticle deposition rhythm persisted in cryb and cryOUT mutant flies, indicating that CRY does not play a core role in the rhythm generation in the epidermal oscillator.

AB - Insect endocuticle thickens after adult emergence by daily alternating deposition of two chitin layers with different orientation. Although the cuticle deposition rhythm is known to be controlled by a circadian clock in many insects, the site of the driving clock, the photoreceptor for entrainment, and the oscillatory mechanism remain elusive. Here, we show that the cuticle deposition rhythm is regulated by a peripheral oscillator in the epidermis in Drosophila melanogaster. Free-running and entrainment experiments in vitro reveal that the oscillator for the cuticle deposition rhythm is independent of the central clock in the brain driving the locomotor rhythms. The cuticle deposition rhythm is absent in null and dominant-negative mutants of clock genes (i.e., period, timeless, cycle, and Clock), indicating that this oscillator is composed of the same clock genes as the central clock. Entrainment experiments with monochromatic light-dark cycles and cryb flies reveal that a blue light-absorbing photoreceptor, cryptochrome (CRY), acts as a photoreceptor pigment for the entrainment of the cuticle deposition rhythm. Unlike other peripheral rhythms in D. melanogaster, the cuticle deposition rhythm persisted in cryb and cryOUT mutant flies, indicating that CRY does not play a core role in the rhythm generation in the epidermal oscillator.

KW - Circadian rhythm

KW - Clock genes

KW - Cryptochrome

KW - Entrainment

KW - Epidermal cell

UR - http://www.scopus.com/inward/record.url?scp=46149100504&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=46149100504&partnerID=8YFLogxK

U2 - 10.1073/pnas.0800145105

DO - 10.1073/pnas.0800145105

M3 - Article

C2 - 18539772

AN - SCOPUS:46149100504

VL - 105

SP - 8446

EP - 8451

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 24

ER -