Peripheral blood circulating immature cell counts predict CD34+ cell yields in G-CSF-induced PBPC mobilization in healthy donors

Teruhiko Kozuka, Kazuma Ikeda, Takanori Teshima, Chikamasa Yoshida, Katsuji Shinagawa, Kensuke Kojima, Keitaro Matsuo, Akihiro Bessho, Kazutaka Sunami, Yasushi Hiramatsu, Yoshinobu Maeda, Toshio Noguchi, Kazuhiko Yamamoto, Nobuharu Fujii, Toshi Imai, Kinuyo Kaneda Kusumoto, Kozo Masuda, Katsuto Takenaka, Fumihiko Ishimaru, Kenji NiiyaNorio Koide, Mitsune Tanimoto, Mine Harada

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18 Citations (Scopus)

Abstract

BACKGROUND: It has been previously reported that the number of circulating immature cells (CIC) in peripheral blood (PB) estimates the number of CD34+ cells collected in G-CSF plus chemotherapy-induced PBPC mobilization. The correlation of CIC counts in PB with CD34+ cell yield and its usefulness was evaluated in G-CSF-induced PBPC mobilization for healthy donors. STUDY DESIGN AND METHODS: CIC counts in PB and CD34+ cell counts in the apheresis product from 122 collections were assessed, and the relationship between these two variables was evaluated with the Pearson rank correlation analysis, the chi-squared test, and the U-test. RESULTS: CIC counts were correlated weakly with the number of CD34+ cells per L of blood processed in the apheresis product (Pearson rank correlation analysis; r = 0.357, p < 0.0001). When a level of 1.7 x 109 CICs per L was selected as a cutoff value, the sensitivity and specificity for collecting more than 20 × 106 CD34+ cells per L of blood processed were 63.6 and 77.5 percent, respectively. CONCLUSION: The present study suggests that the number of CICs in PB may estimate the number of CD34+ cells collected. The data indicate that CIC counts above 1.7 × 109 per L can be used as a good predictor for PBPC collections containing more than 20 × 106 CD34+ cells per L of blood processed in a single apheresis procedure.

Original languageEnglish
Pages (from-to)526-532
Number of pages7
JournalTransfusion
Volume44
Issue number4
DOIs
Publication statusPublished - Apr 1 2004

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

Cite this

Kozuka, T., Ikeda, K., Teshima, T., Yoshida, C., Shinagawa, K., Kojima, K., Matsuo, K., Bessho, A., Sunami, K., Hiramatsu, Y., Maeda, Y., Noguchi, T., Yamamoto, K., Fujii, N., Imai, T., Kusumoto, K. K., Masuda, K., Takenaka, K., Ishimaru, F., ... Harada, M. (2004). Peripheral blood circulating immature cell counts predict CD34+ cell yields in G-CSF-induced PBPC mobilization in healthy donors. Transfusion, 44(4), 526-532. https://doi.org/10.1111/j.1537-2995.2004.03078.x