Periodontitis-induced lipid peroxidation in rat descending aorta is involved in the initiation of atherosclerosis

Daisuke Ekuni, T. Tomofuji, T. Sanbe, K. Irie, T. Azuma, Takayuki Maruyama, N. Tamaki, Jun Murakami, Susumu Kokeguchi, T. Yamamoto

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Background and Objective: Periodontitis is a risk factor for the development of atherosclerosis. Recent studies indicate that oxidative mechanisms, including lipid peroxidation, are involved not only in periodontitis but also in atherosclerosis. Lipid peroxidation may play an important role in the pathogenesis of atherosclerosis, particularly during its earliest stages. The purpose of this study was to investigate the relationship between lipid peroxidation induced by periodontitis and the initiation of atherosclerosis. Material and Methods: Sixteen rats were randomly divided into two groups of eight rats each. Periodontitis was ligature-induced for 4 wk in the experimental group, whereas the control group was left untreated. After the experimental period, the mandibular first molar regions were resected and then subjected to histological analysis and measurement of hexanoyl-lysine expression as an indicator of lipid peroxidation. Descending aorta was used for measuring the levels of hexanoyl-lysine, reactive oxygen species and lipid deposits, and for real-time polymerase chain reaction microarray analysis. The level of hexanoyl-lysine was also measured in serum. Results: In the experimental group, the levels of hexanoyl-lysine in periodontal tissue and serum increased. Only aorta samples in the experimental group showed lipid accumulation, with increased expression of hexanoyl-lysine, reactive oxygen species and oxidative stress-related genes (including nitric oxide synthases 2 and 3), whereas the superoxide dismutase 1 gene level was down-regulated. Conclusion: In a ligature-induced periodontitis rat model, increased lipid peroxidation was found in serum and aorta as well as in periodontal tissue. Atherosclerosis-related gene expression and histological changes were also stimulated. Periodontitis-induced lipid peroxidation in the aorta may be involved in the early stage of atherosclerosis.

Original languageEnglish
Pages (from-to)434-442
Number of pages9
JournalJournal of Periodontal Research
Volume44
Issue number4
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Periodontitis
Thoracic Aorta
Lipid Peroxidation
Atherosclerosis
Lysine
Aorta
Ligation
Reactive Oxygen Species
Serum
Lipids
Microarray Analysis
Nitric Oxide Synthase
Genes
Real-Time Polymerase Chain Reaction
Oxidative Stress
Gene Expression
Control Groups

Keywords

  • Animal model
  • Atherosclerosis
  • Descending aorta
  • Lipid peroxidation
  • Oxidative stress
  • Periodontal disease

ASJC Scopus subject areas

  • Periodontics

Cite this

Periodontitis-induced lipid peroxidation in rat descending aorta is involved in the initiation of atherosclerosis. / Ekuni, Daisuke; Tomofuji, T.; Sanbe, T.; Irie, K.; Azuma, T.; Maruyama, Takayuki; Tamaki, N.; Murakami, Jun; Kokeguchi, Susumu; Yamamoto, T.

In: Journal of Periodontal Research, Vol. 44, No. 4, 08.2009, p. 434-442.

Research output: Contribution to journalArticle

Ekuni, Daisuke ; Tomofuji, T. ; Sanbe, T. ; Irie, K. ; Azuma, T. ; Maruyama, Takayuki ; Tamaki, N. ; Murakami, Jun ; Kokeguchi, Susumu ; Yamamoto, T. / Periodontitis-induced lipid peroxidation in rat descending aorta is involved in the initiation of atherosclerosis. In: Journal of Periodontal Research. 2009 ; Vol. 44, No. 4. pp. 434-442.
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abstract = "Background and Objective: Periodontitis is a risk factor for the development of atherosclerosis. Recent studies indicate that oxidative mechanisms, including lipid peroxidation, are involved not only in periodontitis but also in atherosclerosis. Lipid peroxidation may play an important role in the pathogenesis of atherosclerosis, particularly during its earliest stages. The purpose of this study was to investigate the relationship between lipid peroxidation induced by periodontitis and the initiation of atherosclerosis. Material and Methods: Sixteen rats were randomly divided into two groups of eight rats each. Periodontitis was ligature-induced for 4 wk in the experimental group, whereas the control group was left untreated. After the experimental period, the mandibular first molar regions were resected and then subjected to histological analysis and measurement of hexanoyl-lysine expression as an indicator of lipid peroxidation. Descending aorta was used for measuring the levels of hexanoyl-lysine, reactive oxygen species and lipid deposits, and for real-time polymerase chain reaction microarray analysis. The level of hexanoyl-lysine was also measured in serum. Results: In the experimental group, the levels of hexanoyl-lysine in periodontal tissue and serum increased. Only aorta samples in the experimental group showed lipid accumulation, with increased expression of hexanoyl-lysine, reactive oxygen species and oxidative stress-related genes (including nitric oxide synthases 2 and 3), whereas the superoxide dismutase 1 gene level was down-regulated. Conclusion: In a ligature-induced periodontitis rat model, increased lipid peroxidation was found in serum and aorta as well as in periodontal tissue. Atherosclerosis-related gene expression and histological changes were also stimulated. Periodontitis-induced lipid peroxidation in the aorta may be involved in the early stage of atherosclerosis.",
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AU - Ekuni, Daisuke

AU - Tomofuji, T.

AU - Sanbe, T.

AU - Irie, K.

AU - Azuma, T.

AU - Maruyama, Takayuki

AU - Tamaki, N.

AU - Murakami, Jun

AU - Kokeguchi, Susumu

AU - Yamamoto, T.

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N2 - Background and Objective: Periodontitis is a risk factor for the development of atherosclerosis. Recent studies indicate that oxidative mechanisms, including lipid peroxidation, are involved not only in periodontitis but also in atherosclerosis. Lipid peroxidation may play an important role in the pathogenesis of atherosclerosis, particularly during its earliest stages. The purpose of this study was to investigate the relationship between lipid peroxidation induced by periodontitis and the initiation of atherosclerosis. Material and Methods: Sixteen rats were randomly divided into two groups of eight rats each. Periodontitis was ligature-induced for 4 wk in the experimental group, whereas the control group was left untreated. After the experimental period, the mandibular first molar regions were resected and then subjected to histological analysis and measurement of hexanoyl-lysine expression as an indicator of lipid peroxidation. Descending aorta was used for measuring the levels of hexanoyl-lysine, reactive oxygen species and lipid deposits, and for real-time polymerase chain reaction microarray analysis. The level of hexanoyl-lysine was also measured in serum. Results: In the experimental group, the levels of hexanoyl-lysine in periodontal tissue and serum increased. Only aorta samples in the experimental group showed lipid accumulation, with increased expression of hexanoyl-lysine, reactive oxygen species and oxidative stress-related genes (including nitric oxide synthases 2 and 3), whereas the superoxide dismutase 1 gene level was down-regulated. Conclusion: In a ligature-induced periodontitis rat model, increased lipid peroxidation was found in serum and aorta as well as in periodontal tissue. Atherosclerosis-related gene expression and histological changes were also stimulated. Periodontitis-induced lipid peroxidation in the aorta may be involved in the early stage of atherosclerosis.

AB - Background and Objective: Periodontitis is a risk factor for the development of atherosclerosis. Recent studies indicate that oxidative mechanisms, including lipid peroxidation, are involved not only in periodontitis but also in atherosclerosis. Lipid peroxidation may play an important role in the pathogenesis of atherosclerosis, particularly during its earliest stages. The purpose of this study was to investigate the relationship between lipid peroxidation induced by periodontitis and the initiation of atherosclerosis. Material and Methods: Sixteen rats were randomly divided into two groups of eight rats each. Periodontitis was ligature-induced for 4 wk in the experimental group, whereas the control group was left untreated. After the experimental period, the mandibular first molar regions were resected and then subjected to histological analysis and measurement of hexanoyl-lysine expression as an indicator of lipid peroxidation. Descending aorta was used for measuring the levels of hexanoyl-lysine, reactive oxygen species and lipid deposits, and for real-time polymerase chain reaction microarray analysis. The level of hexanoyl-lysine was also measured in serum. Results: In the experimental group, the levels of hexanoyl-lysine in periodontal tissue and serum increased. Only aorta samples in the experimental group showed lipid accumulation, with increased expression of hexanoyl-lysine, reactive oxygen species and oxidative stress-related genes (including nitric oxide synthases 2 and 3), whereas the superoxide dismutase 1 gene level was down-regulated. Conclusion: In a ligature-induced periodontitis rat model, increased lipid peroxidation was found in serum and aorta as well as in periodontal tissue. Atherosclerosis-related gene expression and histological changes were also stimulated. Periodontitis-induced lipid peroxidation in the aorta may be involved in the early stage of atherosclerosis.

KW - Animal model

KW - Atherosclerosis

KW - Descending aorta

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KW - Oxidative stress

KW - Periodontal disease

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