Perineuronal Sulfated Proteoglycans and Cell Surface Glycoproteins in the Visual Cortex of Adult and Newborn Cats

Sayoko Hitomi, Wei Dong Su, Luo Jia Hong, Aiji Ohtsuka, Takuro Murakami

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Sections of the visual cortex of newborn (1-4 weeks after birth) and adult cats were stained with cationic iron colloid, aldehyde fuchsin or lectins (lectin Vicia villosa, soybean and Wisteria floribunda agglutinins). Many neurons in the adult cat visual cortex contained perineuronal sulfated proteoglycans detectable with cationic iron colloid and aldehyde fuchsin, or cell surface glycoproteins reactive to lectins. Double staining indicated that some of the lectin-labeled neurons were not stained with cationic iron colloid, and also that some of the cationic iron colloid-stained neurons were not labeled with lectins. The perineuronal sulfated proteoglycans and cell surface glycoproteins developed 3 weeks after birth. In the newborn cats 1-2 weeks after birth, no neurons were reactive to cationic iron colloid, aldehyde fuchsin or lectins. In the newborn cats 34 weeks after birth, it was clearly observed that the cytoplasm of the glial cells closely associated with the neurons containing the perineuronal sulfated proteoglycans showed an intense reaction to cationic iron colloid and aldehyde fuchsin, and that the Golgi complexes of the neurons with cell surface glycoproteins were intensely labeled with lectins. These findings suggest that the perineuronal sulfated proteoglycans are derived from the associated glial cells, and that the cell surface glycoproteins are produced by the associated nerve cells.

Original languageEnglish
Pages (from-to)295-299
Number of pages5
JournalActa Medica Okayama
Volume51
Issue number6
Publication statusPublished - Dec 1997

Fingerprint

Membrane Glycoproteins
Visual Cortex
Proteoglycans
Colloids
Neurons
Lectins
Cats
Rosaniline Dyes
Iron
Aldehydes
Parturition
Neuroglia
Golgi Apparatus
Soybeans
Cytoplasm
Staining and Labeling

Keywords

  • Aldehyde fuchsin
  • Cat brain
  • Cationic iron colloid
  • Cell surface glycoproteins
  • Lectin
  • Perineuronal sulfated proteoglycans

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Perineuronal Sulfated Proteoglycans and Cell Surface Glycoproteins in the Visual Cortex of Adult and Newborn Cats. / Hitomi, Sayoko; Su, Wei Dong; Hong, Luo Jia; Ohtsuka, Aiji; Murakami, Takuro.

In: Acta Medica Okayama, Vol. 51, No. 6, 12.1997, p. 295-299.

Research output: Contribution to journalArticle

Hitomi, Sayoko ; Su, Wei Dong ; Hong, Luo Jia ; Ohtsuka, Aiji ; Murakami, Takuro. / Perineuronal Sulfated Proteoglycans and Cell Surface Glycoproteins in the Visual Cortex of Adult and Newborn Cats. In: Acta Medica Okayama. 1997 ; Vol. 51, No. 6. pp. 295-299.
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