Perineuronal nets of proteoglycans in the adult mouse brain, with special reference to their reactions to Gomori's ammoniacal silver and Ehrlich's methylene blue

Takuro Murakami, Tetsuro Murakami, Hiroyuki Sato, Wafaa Alaa El Din Mubarak, Aiji Ohtsuka, Koji Abe

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13 Citations (Scopus)

Abstract

As our previous studies have indicated, many subsets of neurons in the vertebrate brain possess a sulfated proteoglycan surface coat which reacts to cationic iron colloid and aldehyde fuchsin. The present study demonstrated that this surface coat is supravitally stained with Ehrlich's methylene blue, and doubly with this blue and aldehyde fuchsin, a finding suggesting its being identical to Cajal's superficial reticulum (red superficial) and to Golgi's reticular coating (revetement reticulare). The perineuronal surface coat was further stained with Gomori's ammoniacal silver, and doubly with this silver and cationic iron colloid. These neurons with such a proteoglycan surface coat usually expressed cell surface glycoproteins which were labeled with lectin Wisteria floribunda agglutinin. Hyaluronidase digestion did not interfere with this lectin labeling of the glycoproteins, methylene blue and Gomori's ammoniacal silver staining of the surface coat, while it erased the cationic iron colloid and aldehyde fuchsin staining of the surface coat. These findings suggest that the perineuronal proteoglycan surface coat is associated with some additional molecules which are resistant to hyaluronidase digestion and stainable with methylene blue and Gomori's ammoniacal silver. The possibility is suggested that these molecules might represent 'ligand proteoglycans' connecting the perineuronal proteoglycans and cell surface glycoproteins.

Original languageEnglish
Pages (from-to)71-81
Number of pages11
JournalArchives of Histology and Cytology
Volume62
Issue number1
DOIs
Publication statusPublished - Mar 1999

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Methylene Blue
Proteoglycans
Rosaniline Dyes
Colloids
Aldehydes
Brain
Hyaluronoglucosaminidase
Iron
Membrane Glycoproteins
Lectins
Digestion
Neurons
Reticulum
Silver Staining
Silver
Vertebrates
Glycoproteins
ammoniacal silver
Staining and Labeling
Ligands

ASJC Scopus subject areas

  • Anatomy
  • Histology

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Perineuronal nets of proteoglycans in the adult mouse brain, with special reference to their reactions to Gomori's ammoniacal silver and Ehrlich's methylene blue. / Murakami, Takuro; Murakami, Tetsuro; Sato, Hiroyuki; Mubarak, Wafaa Alaa El Din; Ohtsuka, Aiji; Abe, Koji.

In: Archives of Histology and Cytology, Vol. 62, No. 1, 03.1999, p. 71-81.

Research output: Contribution to journalArticle

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