Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice

Ko Miyoshi, Kyosuke Kasahara, Ikuko Miyazaki, Shoko Shimizu, Manabu Taniguchi, Shinsuke Matsuzaki, Masaya Tohyama, Masato Asanuma

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The Drosophila pericentrin-like protein has been shown to be essential for the formation of the sensory cilia of chemosensory and mechanosensory neurons by mutant analysis in flies, while the in vivo function of pericentrin, a well-studied mammalian centrosomal protein related to microcephalic primordial dwarfism, has been unclear. To determine whether pericentrin is required for ciliogenesis in mammals, we generated and analyzed mice with a hypomorphic mutation of Pcnt encoding the mouse pericentrin. Immunofluorescence analysis demonstrated that olfactory cilia of chemosensory neurons in the nasal olfactory epithelium were malformed in the homozygous mutant mice. On the other hand, the assembly of motile and primary cilia of non-neuronal epithelial cells and the formation of sperm flagella were not affected in the Pcnt-mutant mice. The defective assembly of olfactory cilia in the mutant was apparent from birth. The mutant animals displayed reduced olfactory performance in agreement with the compromised assembly of olfactory cilia. Our findings suggest that pericentrin is essential for the assembly of chemosensory cilia of olfactory receptor neurons, but it is not globally required for cilia formation in mammals.

Original languageEnglish
Pages (from-to)3289-3297
Number of pages9
JournalFASEB Journal
Volume23
Issue number10
DOIs
Publication statusPublished - Oct 2009

Fingerprint

Dwarfism
Cilia
Neurons
Mammals
Proteins
Odorant Receptors
Olfactory Receptor Neurons
Animals
Sperm Tail
Olfactory Mucosa
Nasal Mucosa
pericentrin
Diptera
Fluorescent Antibody Technique
Epithelial Cells
Parturition
Mutation

Keywords

  • Ciliogenesis
  • Kendrin
  • Olfaction

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology

Cite this

Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice. / Miyoshi, Ko; Kasahara, Kyosuke; Miyazaki, Ikuko; Shimizu, Shoko; Taniguchi, Manabu; Matsuzaki, Shinsuke; Tohyama, Masaya; Asanuma, Masato.

In: FASEB Journal, Vol. 23, No. 10, 10.2009, p. 3289-3297.

Research output: Contribution to journalArticle

Miyoshi, Ko ; Kasahara, Kyosuke ; Miyazaki, Ikuko ; Shimizu, Shoko ; Taniguchi, Manabu ; Matsuzaki, Shinsuke ; Tohyama, Masaya ; Asanuma, Masato. / Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice. In: FASEB Journal. 2009 ; Vol. 23, No. 10. pp. 3289-3297.
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