Performance of filters in medical protein separation system using superconducting magnet

Hiroshi Ueda, Koh Agatsuma, Mitsuho Furuse, Shuichiro Fuchino, Kazuhiro Kajikawa, Yasuharu Kamioka, Tomohiro Iitsuka, Shuichi Nakamura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Medical proteins such as monoclonal antibodies or immunoglobulin are important as medicine for cancer and other uses. Today, we can easily sort and analyze medical proteins using various types of commercially available affinity magnetic beads. However, separation systems for these medical proteins have a very low separation rate, and the cost of the product is extremely high. We successfully developed a high gradient magnetic separation system using a cryocooler-cooled low-temperature superconducting magnet and conducted experiments on separating affinity magnetic nanobeads. Our system demonstrated very high separation efficiency and can achieve low costs with a large production rate compared to systems now used in this field. The design of a filter to trap and recover the nanobeads is important to this application. In order to achieve a filter with a high trapping ratio of magnetic beads, the parameters need to be optimized because the ratio depends on the dimensions and arrangement of the filter made of fine magnetic metal fibers. In this study, we investigated the performance of filters in the high gradientmagnetic separation system. The test results show 97.8% of the magnetic nanobeads in pure water were captured, and 94.1% of the total beads were collected.

Original languageEnglish
Article number6678093
JournalIEEE Transactions on Applied Superconductivity
Volume24
Issue number3
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Superconducting magnets
superconducting magnets
proteins
Proteins
filters
beads
affinity
Magnetic separation
Monoclonal antibodies
metal fibers
magnetic metals
Medicine
Immunoglobulins
Costs
antibodies
medicine
Metals
Monoclonal Antibodies
cancer
trapping

Keywords

  • Affinity magnetic nanobeads
  • High-gradientmagnetic separation
  • Immunomagnetic cell sorting
  • Magnetic filter

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Performance of filters in medical protein separation system using superconducting magnet. / Ueda, Hiroshi; Agatsuma, Koh; Furuse, Mitsuho; Fuchino, Shuichiro; Kajikawa, Kazuhiro; Kamioka, Yasuharu; Iitsuka, Tomohiro; Nakamura, Shuichi.

In: IEEE Transactions on Applied Superconductivity, Vol. 24, No. 3, 6678093, 2014.

Research output: Contribution to journalArticle

Ueda, H, Agatsuma, K, Furuse, M, Fuchino, S, Kajikawa, K, Kamioka, Y, Iitsuka, T & Nakamura, S 2014, 'Performance of filters in medical protein separation system using superconducting magnet', IEEE Transactions on Applied Superconductivity, vol. 24, no. 3, 6678093. https://doi.org/10.1109/TASC.2013.2290374
Ueda, Hiroshi ; Agatsuma, Koh ; Furuse, Mitsuho ; Fuchino, Shuichiro ; Kajikawa, Kazuhiro ; Kamioka, Yasuharu ; Iitsuka, Tomohiro ; Nakamura, Shuichi. / Performance of filters in medical protein separation system using superconducting magnet. In: IEEE Transactions on Applied Superconductivity. 2014 ; Vol. 24, No. 3.
@article{6332ce01021c49da8e370583edb15b16,
title = "Performance of filters in medical protein separation system using superconducting magnet",
abstract = "Medical proteins such as monoclonal antibodies or immunoglobulin are important as medicine for cancer and other uses. Today, we can easily sort and analyze medical proteins using various types of commercially available affinity magnetic beads. However, separation systems for these medical proteins have a very low separation rate, and the cost of the product is extremely high. We successfully developed a high gradient magnetic separation system using a cryocooler-cooled low-temperature superconducting magnet and conducted experiments on separating affinity magnetic nanobeads. Our system demonstrated very high separation efficiency and can achieve low costs with a large production rate compared to systems now used in this field. The design of a filter to trap and recover the nanobeads is important to this application. In order to achieve a filter with a high trapping ratio of magnetic beads, the parameters need to be optimized because the ratio depends on the dimensions and arrangement of the filter made of fine magnetic metal fibers. In this study, we investigated the performance of filters in the high gradientmagnetic separation system. The test results show 97.8{\%} of the magnetic nanobeads in pure water were captured, and 94.1{\%} of the total beads were collected.",
keywords = "Affinity magnetic nanobeads, High-gradientmagnetic separation, Immunomagnetic cell sorting, Magnetic filter",
author = "Hiroshi Ueda and Koh Agatsuma and Mitsuho Furuse and Shuichiro Fuchino and Kazuhiro Kajikawa and Yasuharu Kamioka and Tomohiro Iitsuka and Shuichi Nakamura",
year = "2014",
doi = "10.1109/TASC.2013.2290374",
language = "English",
volume = "24",
journal = "IEEE Transactions on Applied Superconductivity",
issn = "1051-8223",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
number = "3",

}

TY - JOUR

T1 - Performance of filters in medical protein separation system using superconducting magnet

AU - Ueda, Hiroshi

AU - Agatsuma, Koh

AU - Furuse, Mitsuho

AU - Fuchino, Shuichiro

AU - Kajikawa, Kazuhiro

AU - Kamioka, Yasuharu

AU - Iitsuka, Tomohiro

AU - Nakamura, Shuichi

PY - 2014

Y1 - 2014

N2 - Medical proteins such as monoclonal antibodies or immunoglobulin are important as medicine for cancer and other uses. Today, we can easily sort and analyze medical proteins using various types of commercially available affinity magnetic beads. However, separation systems for these medical proteins have a very low separation rate, and the cost of the product is extremely high. We successfully developed a high gradient magnetic separation system using a cryocooler-cooled low-temperature superconducting magnet and conducted experiments on separating affinity magnetic nanobeads. Our system demonstrated very high separation efficiency and can achieve low costs with a large production rate compared to systems now used in this field. The design of a filter to trap and recover the nanobeads is important to this application. In order to achieve a filter with a high trapping ratio of magnetic beads, the parameters need to be optimized because the ratio depends on the dimensions and arrangement of the filter made of fine magnetic metal fibers. In this study, we investigated the performance of filters in the high gradientmagnetic separation system. The test results show 97.8% of the magnetic nanobeads in pure water were captured, and 94.1% of the total beads were collected.

AB - Medical proteins such as monoclonal antibodies or immunoglobulin are important as medicine for cancer and other uses. Today, we can easily sort and analyze medical proteins using various types of commercially available affinity magnetic beads. However, separation systems for these medical proteins have a very low separation rate, and the cost of the product is extremely high. We successfully developed a high gradient magnetic separation system using a cryocooler-cooled low-temperature superconducting magnet and conducted experiments on separating affinity magnetic nanobeads. Our system demonstrated very high separation efficiency and can achieve low costs with a large production rate compared to systems now used in this field. The design of a filter to trap and recover the nanobeads is important to this application. In order to achieve a filter with a high trapping ratio of magnetic beads, the parameters need to be optimized because the ratio depends on the dimensions and arrangement of the filter made of fine magnetic metal fibers. In this study, we investigated the performance of filters in the high gradientmagnetic separation system. The test results show 97.8% of the magnetic nanobeads in pure water were captured, and 94.1% of the total beads were collected.

KW - Affinity magnetic nanobeads

KW - High-gradientmagnetic separation

KW - Immunomagnetic cell sorting

KW - Magnetic filter

UR - http://www.scopus.com/inward/record.url?scp=84990877527&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84990877527&partnerID=8YFLogxK

U2 - 10.1109/TASC.2013.2290374

DO - 10.1109/TASC.2013.2290374

M3 - Article

AN - SCOPUS:84990877527

VL - 24

JO - IEEE Transactions on Applied Superconductivity

JF - IEEE Transactions on Applied Superconductivity

SN - 1051-8223

IS - 3

M1 - 6678093

ER -