Perception of the chitin oligosaccharides contributes to disease resistance to blast fungus Magnaporthe oryzae in rice

Kyutaro Kishimoto, Yusuke Kouzai, Hanae Kaku, Naoto Shibuya, Eiichi Minami, Yoko Nishizawa

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Chitin is a component of fungal cell walls, and its fragments act as elicitors in many plants. The plasma membrane glycoprotein CEBiP, which possesses LysM domains, is a receptor for the chitin elicitor (CE) in rice. Here, we report that the perception of CE by CEBiP contributes to disease resistance against the rice blast fungus, Magnaporthe oryzae, and that enhanced responses to CE by engineering CEBiP increase disease tolerance. Knockdown of CEBiP expression allowed increased spread of the infection hyphae. To enhance defense responses to CE, we constructed chimeric genes composed of CEBiP and Xa21, which mediate resistance to rice bacterial leaf blight. The expression of either CRXal or CFtXa3, each of which contains the whole extracellular portion of CEBiP, the whole intracellular domain of XA21, and the transmembrane domain from either CEBiP or XA21, induced cell death accompanied by an increased production of reactive oxygen and nitrogen species after treatment with CE. Rice plants expressing the chimeric receptor exhibited necrotic lesions in response to CE and became more resistant to M. oryzae. Deletion of the first LysM domain in CRXA1 abolished these cellular responses. These results suggest that CEs are produced and recognized through the LysM domain of CEBiP during the interaction between rice and M. oryzae and imply that engineering pattern recognition receptors represents a new strategy for crop protection against fungal diseases.

Original languageEnglish
Pages (from-to)343-354
Number of pages12
JournalPlant Journal
Volume64
Issue number2
DOIs
Publication statusPublished - Oct 2010
Externally publishedYes

Fingerprint

Magnaporthe
Magnaporthe oryzae
Disease Resistance
Chitin
chitin
Oligosaccharides
oligosaccharides
disease resistance
Fungi
rice
fungi
receptors
engineering
membrane glycoproteins
Fungal Structures
lesions (plant)
leaf blight
blast disease
Pattern Recognition Receptors
Reactive Nitrogen Species

Keywords

  • Chimeric receptor
  • Chitin signal
  • Disease resistance
  • HR cell death
  • MAMP-triggered immunity
  • Rice blast fungus

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

Cite this

Perception of the chitin oligosaccharides contributes to disease resistance to blast fungus Magnaporthe oryzae in rice. / Kishimoto, Kyutaro; Kouzai, Yusuke; Kaku, Hanae; Shibuya, Naoto; Minami, Eiichi; Nishizawa, Yoko.

In: Plant Journal, Vol. 64, No. 2, 10.2010, p. 343-354.

Research output: Contribution to journalArticle

Kishimoto, Kyutaro ; Kouzai, Yusuke ; Kaku, Hanae ; Shibuya, Naoto ; Minami, Eiichi ; Nishizawa, Yoko. / Perception of the chitin oligosaccharides contributes to disease resistance to blast fungus Magnaporthe oryzae in rice. In: Plant Journal. 2010 ; Vol. 64, No. 2. pp. 343-354.
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