Peptide mimics of monocyte chemoattractant protein-1 (MCP-1) with an antagonistic activity

Masatomo Kaji, Masaomi Ikari, Shuhei Hashiguchi, Yuji Ito, Ryo Matsumoto, Teizo Yoshimura, Jun Ichi Kuratsu, Kazuhisa Sugimura

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In this study, we attempted to analyze the peptide motifs recognized by 24822.111 and F9, monoclonal antibodies (mAbs) that inhibit the chemotactic activity of monocyte chemoattractant protein-1 (MCP-1), a member of the CC subfamily of chemokines. We isolated phage clones from a phage display library and identified six peptide motifs. One of these clones, C27, was strongly and specifically recognized by 24822.111 mAb, while another, G25, was similarly recognized by F9 mAb. Both the C27 motif and the G25 motif contain two cysteines in their sequences and have little homology to the primary amino acid sequence of MCP-1. These clones, however, bound to THP-1 cells, and the binding was competitively inhibited by MCP-1. The clones strongly inhibited the MCP-1-induced chemotaxis of human monocytes. The synthetic and intramolecularly disulfide-linked peptides of C27 and G25 (sC27 and sG25) also inhibited the chemotaxis induced by MCP-1, while their derivatives with serine in place of cysteine did not, suggesting the importance of the loop structure for the inhibition. These results suggest that sC27 and sG25 may mimic the MCP-1-binding domain to the MCP-1 receptor.

Original languageEnglish
Pages (from-to)577-583
Number of pages7
JournalJournal of biochemistry
Volume129
Issue number4
DOIs
Publication statusPublished - Apr 2001

Keywords

  • Inhibition
  • MCP-1
  • Molecular design
  • Peptide mimic
  • Phage library

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Kaji, M., Ikari, M., Hashiguchi, S., Ito, Y., Matsumoto, R., Yoshimura, T., Kuratsu, J. I., & Sugimura, K. (2001). Peptide mimics of monocyte chemoattractant protein-1 (MCP-1) with an antagonistic activity. Journal of biochemistry, 129(4), 577-583. https://doi.org/10.1093/oxfordjournals.jbchem.a002893