Abstract
Peptide 19-immunoreactivity (PEP 19-ir) was examined in the dorsal root ganglion (DRG) and the mesencephalic trigeminal tract nucleus (Mes5) of the adult rat. Thirty-eight percent of DRG cells were immunoreactive (ir) for PEP 19. These neurons were small to large and measured 167-4583 μm2 (mean ± S.D. = 2048 ± 913 μm2). Seventy-five percent of DRG cells > 2000 μm2 and 15% of those <1000 μm2 exhibited PEP 19-ir. Thirty-six percent of DRG neurons in the range 1000-2000 μm2 showed the ir. In the Mes5, 87% of primary neurons were ir for this peptide. Muscle spindles in the soleus and masseter muscles contained PEP 19-ir spiral axon terminals. Double immunofluorescence methods revealed the co-expression of PEP 19 and calcium binding proteins. Eighty-six percent of parvalbumin-ir neurons exhibited PEP 19-ir. Conversely, 60% of PEP 19-ir neurons showed parvalbumin-ir. The cell size analysis revealed that 55% of PEP 19-ir neurons > 600 μm2 showed parvalbumin-ir and that all PEP 19-ir neurons <600 μm2 were devoid of it. Ninety percent of PEP 19-ir DRG neurons showed S100-ir, whereas 60% of S100- ir ones co-expressed PEP 19-ir. In the Mes5, virtually all PEP 19-ir primary neurons exhibited parvalbumin-ir. The co-expression of PEP 19 and S100 could not be observed in the nucleus. The present study indicates that PEP 19-ir neurons which co-express parvalbumin-ir are proprioceptors in the spinal and the trigeminal systems. PEP 19-ir small DRG neurons without S100-ir are probably exteroceptors and may include unmyelinated nociceptors.
| Original language | English |
|---|---|
| Pages (from-to) | 231-235 |
| Number of pages | 5 |
| Journal | Brain Research |
| Volume | 821 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Mar 6 1999 |
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Keywords
- Dorsal root ganglion
- Mesencephalic trigeminal tract nucleus
- Parvalbumin
- Peptide 19
- Proprioceptor
- S100
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Peptide 19 in the dorsal root ganglion and the mesencephalic trigeminal tract nucleus of the adult rat. / Ichikawa, Hiroyuki; Morgan, James I.; Sugimoto, Tomosada.
In: Brain Research, Vol. 821, No. 1, 06.03.1999, p. 231-235.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Peptide 19 in the dorsal root ganglion and the mesencephalic trigeminal tract nucleus of the adult rat
AU - Ichikawa, Hiroyuki
AU - Morgan, James I.
AU - Sugimoto, Tomosada
PY - 1999/3/6
Y1 - 1999/3/6
N2 - Peptide 19-immunoreactivity (PEP 19-ir) was examined in the dorsal root ganglion (DRG) and the mesencephalic trigeminal tract nucleus (Mes5) of the adult rat. Thirty-eight percent of DRG cells were immunoreactive (ir) for PEP 19. These neurons were small to large and measured 167-4583 μm2 (mean ± S.D. = 2048 ± 913 μm2). Seventy-five percent of DRG cells > 2000 μm2 and 15% of those <1000 μm2 exhibited PEP 19-ir. Thirty-six percent of DRG neurons in the range 1000-2000 μm2 showed the ir. In the Mes5, 87% of primary neurons were ir for this peptide. Muscle spindles in the soleus and masseter muscles contained PEP 19-ir spiral axon terminals. Double immunofluorescence methods revealed the co-expression of PEP 19 and calcium binding proteins. Eighty-six percent of parvalbumin-ir neurons exhibited PEP 19-ir. Conversely, 60% of PEP 19-ir neurons showed parvalbumin-ir. The cell size analysis revealed that 55% of PEP 19-ir neurons > 600 μm2 showed parvalbumin-ir and that all PEP 19-ir neurons <600 μm2 were devoid of it. Ninety percent of PEP 19-ir DRG neurons showed S100-ir, whereas 60% of S100- ir ones co-expressed PEP 19-ir. In the Mes5, virtually all PEP 19-ir primary neurons exhibited parvalbumin-ir. The co-expression of PEP 19 and S100 could not be observed in the nucleus. The present study indicates that PEP 19-ir neurons which co-express parvalbumin-ir are proprioceptors in the spinal and the trigeminal systems. PEP 19-ir small DRG neurons without S100-ir are probably exteroceptors and may include unmyelinated nociceptors.
AB - Peptide 19-immunoreactivity (PEP 19-ir) was examined in the dorsal root ganglion (DRG) and the mesencephalic trigeminal tract nucleus (Mes5) of the adult rat. Thirty-eight percent of DRG cells were immunoreactive (ir) for PEP 19. These neurons were small to large and measured 167-4583 μm2 (mean ± S.D. = 2048 ± 913 μm2). Seventy-five percent of DRG cells > 2000 μm2 and 15% of those <1000 μm2 exhibited PEP 19-ir. Thirty-six percent of DRG neurons in the range 1000-2000 μm2 showed the ir. In the Mes5, 87% of primary neurons were ir for this peptide. Muscle spindles in the soleus and masseter muscles contained PEP 19-ir spiral axon terminals. Double immunofluorescence methods revealed the co-expression of PEP 19 and calcium binding proteins. Eighty-six percent of parvalbumin-ir neurons exhibited PEP 19-ir. Conversely, 60% of PEP 19-ir neurons showed parvalbumin-ir. The cell size analysis revealed that 55% of PEP 19-ir neurons > 600 μm2 showed parvalbumin-ir and that all PEP 19-ir neurons <600 μm2 were devoid of it. Ninety percent of PEP 19-ir DRG neurons showed S100-ir, whereas 60% of S100- ir ones co-expressed PEP 19-ir. In the Mes5, virtually all PEP 19-ir primary neurons exhibited parvalbumin-ir. The co-expression of PEP 19 and S100 could not be observed in the nucleus. The present study indicates that PEP 19-ir neurons which co-express parvalbumin-ir are proprioceptors in the spinal and the trigeminal systems. PEP 19-ir small DRG neurons without S100-ir are probably exteroceptors and may include unmyelinated nociceptors.
KW - Dorsal root ganglion
KW - Mesencephalic trigeminal tract nucleus
KW - Parvalbumin
KW - Peptide 19
KW - Proprioceptor
KW - S100
UR - http://www.scopus.com/inward/record.url?scp=0033528468&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033528468&partnerID=8YFLogxK
U2 - 10.1016/S0006-8993(98)01367-5
DO - 10.1016/S0006-8993(98)01367-5
M3 - Article
C2 - 10064808
AN - SCOPUS:0033528468
VL - 821
SP - 231
EP - 235
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1
ER -