Peplomycin-induced apoptosis in oral squamous carcinoma cells depends on bleomycin sensitivity

Hirohiko Okamura, H. Morimoto, T. Haneji

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Oral squamous carcinoma cell line SSCKN cells were shown to be highly sensitive to bleomycin, whereas SCCTF cells were minimally sensitive to this reagent. To determine whether the anticancer drug resistance to oral squamous carcinoma cells could be related to the degree of the drug-induced apoptosis, we examined the effects of peplomycin on induction of apoptosis in these cells. After reaching subconfluence, SCCKN and SCCTF cells were exposed to various concentrations of peplomycin. Peplomycin caused cytotoxicity in both SCCKN and SCCTF cells in a dose-dependent fashion with the maximal effect at concentrations of 1 and 10 μM, respectively, as determined by phase-contrast microscopy and WST-1 cell viability assay. By using the Hoechst 33342 staining, we observed marked nuclear condensation and fragmentation of chromatin in SCCKN cells treated with 1 μM peplomycin. However, SCCTF cells treated with 1 μM peplomycin showed neither nuclear condensation nor fragmentation. DNA ladder formation was also detected in both cell lines by treatment with peplomycin. The induced DNA ladder formation in SCCKN and SCCTF cells was dose-dependent, with the maximal effect at concentrations of 5 and 50 μM, respectively. Bleomycin also induced DNA ladder formation in SCCKN and SCCTF cells with different sensitivities. Mitomycin C induced DNA laddering in both SCCKN and SCCTF cells; however, the intensity of DNA ladder formation was almost the same in both cell lines. The present results indicate that peplomycin-induced apoptosis in oral squamous carcinoma cell lines depends on the sensitivity of these cells to bleomycin.

Original languageEnglish
Pages (from-to)379-385
Number of pages7
JournalOral Oncology
Volume37
Issue number4
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Peplomycin
Bleomycin
Squamous Cell Carcinoma
Apoptosis
DNA
Cell Line
Phase-Contrast Microscopy
Mitomycin
Drug Resistance
Chromatin

Keywords

  • Apoptosis
  • Bleomycin
  • Peplomycin
  • SCC cells

ASJC Scopus subject areas

  • Oncology

Cite this

Peplomycin-induced apoptosis in oral squamous carcinoma cells depends on bleomycin sensitivity. / Okamura, Hirohiko; Morimoto, H.; Haneji, T.

In: Oral Oncology, Vol. 37, No. 4, 2001, p. 379-385.

Research output: Contribution to journalArticle

@article{855f8f4acaf042839be33a5ee3b7c63c,
title = "Peplomycin-induced apoptosis in oral squamous carcinoma cells depends on bleomycin sensitivity",
abstract = "Oral squamous carcinoma cell line SSCKN cells were shown to be highly sensitive to bleomycin, whereas SCCTF cells were minimally sensitive to this reagent. To determine whether the anticancer drug resistance to oral squamous carcinoma cells could be related to the degree of the drug-induced apoptosis, we examined the effects of peplomycin on induction of apoptosis in these cells. After reaching subconfluence, SCCKN and SCCTF cells were exposed to various concentrations of peplomycin. Peplomycin caused cytotoxicity in both SCCKN and SCCTF cells in a dose-dependent fashion with the maximal effect at concentrations of 1 and 10 μM, respectively, as determined by phase-contrast microscopy and WST-1 cell viability assay. By using the Hoechst 33342 staining, we observed marked nuclear condensation and fragmentation of chromatin in SCCKN cells treated with 1 μM peplomycin. However, SCCTF cells treated with 1 μM peplomycin showed neither nuclear condensation nor fragmentation. DNA ladder formation was also detected in both cell lines by treatment with peplomycin. The induced DNA ladder formation in SCCKN and SCCTF cells was dose-dependent, with the maximal effect at concentrations of 5 and 50 μM, respectively. Bleomycin also induced DNA ladder formation in SCCKN and SCCTF cells with different sensitivities. Mitomycin C induced DNA laddering in both SCCKN and SCCTF cells; however, the intensity of DNA ladder formation was almost the same in both cell lines. The present results indicate that peplomycin-induced apoptosis in oral squamous carcinoma cell lines depends on the sensitivity of these cells to bleomycin.",
keywords = "Apoptosis, Bleomycin, Peplomycin, SCC cells",
author = "Hirohiko Okamura and H. Morimoto and T. Haneji",
year = "2001",
doi = "10.1016/S1368-8375(00)00101-9",
language = "English",
volume = "37",
pages = "379--385",
journal = "Oral Oncology",
issn = "1368-8375",
publisher = "Elsevier Limited",
number = "4",

}

TY - JOUR

T1 - Peplomycin-induced apoptosis in oral squamous carcinoma cells depends on bleomycin sensitivity

AU - Okamura, Hirohiko

AU - Morimoto, H.

AU - Haneji, T.

PY - 2001

Y1 - 2001

N2 - Oral squamous carcinoma cell line SSCKN cells were shown to be highly sensitive to bleomycin, whereas SCCTF cells were minimally sensitive to this reagent. To determine whether the anticancer drug resistance to oral squamous carcinoma cells could be related to the degree of the drug-induced apoptosis, we examined the effects of peplomycin on induction of apoptosis in these cells. After reaching subconfluence, SCCKN and SCCTF cells were exposed to various concentrations of peplomycin. Peplomycin caused cytotoxicity in both SCCKN and SCCTF cells in a dose-dependent fashion with the maximal effect at concentrations of 1 and 10 μM, respectively, as determined by phase-contrast microscopy and WST-1 cell viability assay. By using the Hoechst 33342 staining, we observed marked nuclear condensation and fragmentation of chromatin in SCCKN cells treated with 1 μM peplomycin. However, SCCTF cells treated with 1 μM peplomycin showed neither nuclear condensation nor fragmentation. DNA ladder formation was also detected in both cell lines by treatment with peplomycin. The induced DNA ladder formation in SCCKN and SCCTF cells was dose-dependent, with the maximal effect at concentrations of 5 and 50 μM, respectively. Bleomycin also induced DNA ladder formation in SCCKN and SCCTF cells with different sensitivities. Mitomycin C induced DNA laddering in both SCCKN and SCCTF cells; however, the intensity of DNA ladder formation was almost the same in both cell lines. The present results indicate that peplomycin-induced apoptosis in oral squamous carcinoma cell lines depends on the sensitivity of these cells to bleomycin.

AB - Oral squamous carcinoma cell line SSCKN cells were shown to be highly sensitive to bleomycin, whereas SCCTF cells were minimally sensitive to this reagent. To determine whether the anticancer drug resistance to oral squamous carcinoma cells could be related to the degree of the drug-induced apoptosis, we examined the effects of peplomycin on induction of apoptosis in these cells. After reaching subconfluence, SCCKN and SCCTF cells were exposed to various concentrations of peplomycin. Peplomycin caused cytotoxicity in both SCCKN and SCCTF cells in a dose-dependent fashion with the maximal effect at concentrations of 1 and 10 μM, respectively, as determined by phase-contrast microscopy and WST-1 cell viability assay. By using the Hoechst 33342 staining, we observed marked nuclear condensation and fragmentation of chromatin in SCCKN cells treated with 1 μM peplomycin. However, SCCTF cells treated with 1 μM peplomycin showed neither nuclear condensation nor fragmentation. DNA ladder formation was also detected in both cell lines by treatment with peplomycin. The induced DNA ladder formation in SCCKN and SCCTF cells was dose-dependent, with the maximal effect at concentrations of 5 and 50 μM, respectively. Bleomycin also induced DNA ladder formation in SCCKN and SCCTF cells with different sensitivities. Mitomycin C induced DNA laddering in both SCCKN and SCCTF cells; however, the intensity of DNA ladder formation was almost the same in both cell lines. The present results indicate that peplomycin-induced apoptosis in oral squamous carcinoma cell lines depends on the sensitivity of these cells to bleomycin.

KW - Apoptosis

KW - Bleomycin

KW - Peplomycin

KW - SCC cells

UR - http://www.scopus.com/inward/record.url?scp=0035033075&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035033075&partnerID=8YFLogxK

U2 - 10.1016/S1368-8375(00)00101-9

DO - 10.1016/S1368-8375(00)00101-9

M3 - Article

C2 - 11337271

AN - SCOPUS:0035033075

VL - 37

SP - 379

EP - 385

JO - Oral Oncology

JF - Oral Oncology

SN - 1368-8375

IS - 4

ER -